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Literature summary for 1.3.1.31 extracted from
- Alkene hydrogenation activity of enoate reductases for an environmentally benign biosynthesis of adipic acid (2017), Chem. Sci., 8, 1406-1413 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | hydrogenation activity of enoate reductases can potentially replace the chemical hydrogenation step in current synthetic protocols creating a completely bio-based pathway for the greener production of adipic acid or other biochemicals e.g. 3-phenylpropanoic acid from renewable feedstocks | Clostridium acetobutylicum |
| synthesis | hydrogenation activity of enoate reductases can potentially replace the chemical hydrogenation step in current synthetic protocols creating a completely bio-based pathway for the greener production of adipic acid or other biochemicals e.g. 3-phenylpropanoic acid from renewable feedstocks. High oxygen tolerance and thermostability make the enzyme useful for in vivo and in vitro applications to overcome the limitations of chemical catalysts | Weizmannia coagulans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Weizmannia coagulans |
| expression in Escherichia coli | Clostridium acetobutylicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| trans-cinnamic acid | moderate substrate inhibition | Clostridium acetobutylicum |  |
| trans-cinnamic acid | moderate substrate inhibition | Weizmannia coagulans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
trans-cinnamic acid | pH and temperature not specified in the publication | Clostridium acetobutylicum | |
| 0.68 | - |
trans-cinnamic acid | pH and temperature not specified in the publication | Weizmannia coagulans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acetobutylicum | - |
- |
- |
| Weizmannia coagulans | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| high oxygen tolerance with 33% remaining activity after three days of incubation with air | Weizmannia coagulans |
| in the presence of air (21% oxygen), the enzyme (ER-CA) exhibits 30% residual activity after one day of storage and is completely inactivated by oxygen after two additional days of storage | Clostridium acetobutylicum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Weizmannia coagulans |
- |
Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-cyclohexen-1-one + NADH + H+ | - |
Weizmannia coagulans | ? + NAD+ | - |
? | |
| 2-cyclohexen-1-one + NADH + H+ | - |
Clostridium acetobutylicum | ? + NAD+ | - |
? | |
| 2-hexenedioic acid + NADH + H+ | high conversion rate and yield, no by-products formed | Weizmannia coagulans | adipic acid + NAD+ | - |
? | |
| 2-hexenedioic acid + NADH + H+ | high conversion rate and yield, no by-products formed | Clostridium acetobutylicum | adipic acid + NAD+ | - |
? | |
| cis,cis-muconic acid + NADH + H+ | - |
Weizmannia coagulans | ? + NAD+ | - |
? | |
| cis,cis-muconic acid + NADH + H+ | - |
Clostridium acetobutylicum | ? + NAD+ | - |
? | |
| trans,trans-muconic acid + NADH + H+ | - |
Weizmannia coagulans | ? + NAD+ | - |
? | |
| trans,trans-muconic acid + NADH + H+ | - |
Clostridium acetobutylicum | ? + NAD+ | - |
? | |
| trans-cinnamic acid + NADH + H+ | - |
Weizmannia coagulans | 3-phenylpropanoic acid + NAD+ | - |
? | |
| trans-cinnamic acid + NADH + H+ | - |
Clostridium acetobutylicum | 3-phenylpropanoic acid + NAD+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ER-BC | - |
Weizmannia coagulans |
| ER-CA | - |
Clostridium acetobutylicum |
| OYE | - |
Weizmannia coagulans |
| OYE | - |
Clostridium acetobutylicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 39 | - |
temperature of aggregation | Clostridium acetobutylicum |
| 49.9 | - |
temperature of aggregation | Weizmannia coagulans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
trans-cinnamic acid | pH and temperature not specified in the publication | Weizmannia coagulans | |
| 9.7 | - |
trans-cinnamic acid | pH and temperature not specified in the publication | Clostridium acetobutylicum | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
trans-cinnamic acid | pH and temperature not specified in the publication | Clostridium acetobutylicum | |
| 1.4 | - |
trans-cinnamic acid | pH and temperature not specified in the publication | Weizmannia coagulans | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
trans-cinnamic acid | pH and temperature not specified in the publication | Weizmannia coagulans | |
| 24 | - |
trans-cinnamic acid | pH and temperature not specified in the publication | Clostridium acetobutylicum | |
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