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Literature summary for 1.3.1.31 extracted from

  • Mordaka, P.M.; Hall, S.J.; Minton, N.; Stephens, G.
    Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes (2018), Microbiology, 164, 122-132 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.31

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli with N-terminal His-tag. Successful expression depends on the use of strictly anaerobic conditions for both growth and enzyme preparation Clostridium sporogenes

Organism

Organism UniProt Comment Textmining
Clostridium sporogenes A0A142FJE4
-
-
Clostridium sporogenes DSM 795 A0A142FJE4
-
-

Oxidation Stability

Oxidation Stability Organism
the oxygen-sensitive enzyme loses 57% of the activity after 2 h of exposure to air, and activity can not be restored by reintroduction of anaerobic conditions Clostridium sporogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.174
-
 reductase with the N-terminal His-tag, cinnamic acid as the substrate, pH and temperature not specified in the publication Clostridium sporogenes

Storage Stability

Storage Stability Organism
4°C, anaerobic conditions, no loss of activity Clostridium sporogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-1-nitro-2-phenylpropene + NADH + H+ (E)-1-nitro-2-phenylpropene is reduced to enantiopure (R)-1-nitro-2-phenylpropane with a yield of 90% Clostridium sporogenes (R)-1-nitro-2-phenylpropane + NAD+
-
 ?
(E)-1-nitro-2-phenylpropene + NADH + H+ (E)-1-nitro-2-phenylpropene is reduced to enantiopure (R)-1-nitro-2-phenylpropane with a yield of 90% Clostridium sporogenes DSM 795 (R)-1-nitro-2-phenylpropane + NAD+
-
 ?
(E)-2-nitro-1-phenylpropene + NADH + H+ (E)-2-nitro-1-phenylpropene is reduced with a yield of 56% and enantioselectivity (16% ee for (S)-2-nitro-1-phenylpropane) Clostridium sporogenes (S)-2-nitro-1-phenylpropane + NAD+
-
 ?
(E)-2-nitro-1-phenylpropene + NADH + H+ (E)-2-nitro-1-phenylpropene is reduced with a yield of 56% and enantioselectivity (16% ee for (S)-2-nitro-1-phenylpropane) Clostridium sporogenes DSM 795 (S)-2-nitro-1-phenylpropane + NAD+
-
 ?
cinnamic acid + NADH + H+
-
 Clostridium sporogenes 3-phenylpropionic acid + NAD+
-
 ?
cinnamic acid + NADH + H+
-
 Clostridium sporogenes DSM 795 3-phenylpropionic acid + NAD+
-
 ?
additional information no activity with aliphatic enoates Clostridium sporogenes ?
-
-
additional information no activity with aliphatic enoates Clostridium sporogenes DSM 795 ?
-
-
p-coumaric acid + NADH + H+ the activity is 43% activity when compared to cinnamic acid Clostridium sporogenes ? + NAD+
-
 ?
p-coumaric acid + NADH + H+ the activity is 43% activity when compared to cinnamic acid Clostridium sporogenes DSM 795 ? + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
fldZ
-
 Clostridium sporogenes
NADH-dependent 2-enoate reductase
-
 Clostridium sporogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Clostridium sporogenes

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
32
-
 32°C: about 45% of maximal activity, 47°C: about 50% of maximal activity, completely inactive at 60°C Clostridium sporogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Clostridium sporogenes

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8 pH 6.8: about 75% of maximal activity, pH 8.0: about 80% of maximal activity Clostridium sporogenes

Cofactor

Cofactor Comment Organism Structure
FAD contains an FAD binding sites Clostridium sporogenes
FMN contains an FMN binding sites Clostridium sporogenes
iron-sulfur centre
-
 Clostridium sporogenes