Activating Compound | Comment | Organism | Structure |
---|---|---|---|
histidine | histidine-HCl buffer results in considerably higher activity than corresponding bisTris-HCl or Tris-HCl buffers | Cupriavidus necator |
Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
General Stability | Organism |
---|---|
addition of DTT significantly stabilized enzyme | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-oxoadipate | competitive inhibition | Cupriavidus necator | |
Mn2+ | - |
Cupriavidus necator | |
MnSO4 | - |
Cupriavidus necator | |
p-chloromercuribenzoate | - |
Cupriavidus necator | |
p-chloromercuribenzoate | - |
Cutaneotrichosporon cutaneum | |
p-chloromercuribenzoate | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
NADH | 2-chloromaleylacetate as substrate | Cupriavidus necator | |
0.031 | - |
maleylacetate | - |
Cupriavidus necator | |
0.031 | - |
2-chloromaleylacetate | - |
Cupriavidus necator | |
0.041 | - |
NADH | maleylacetate as substrate | Cupriavidus necator | |
0.082 | - |
NADPH | 2-chloromaleylacetate as substrate | Cupriavidus necator | |
0.089 | - |
NADPH | maleylacetate as substrate | Cupriavidus necator | |
0.09 | - |
maleylacetate | NADPH as cosubstrate | Cupriavidus necator | |
0.132 | - |
2-chloromaleylacetate | NADPH as cosubstrate | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
strain PKO1 | Pseudomonas sp. |
35000 | - |
2 * 35000, SDS-PAGE | Cupriavidus necator |
40000 | - |
2 * 40000, SDS-PAGE | Cutaneotrichosporon cutaneum |
56000 | - |
gel filtration, Superdex 200 | Cupriavidus necator |
72000 | - |
analytical gel filtration | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-maleylacetate + NADPH | Pseudomonas sp. | - |
3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | Cupriavidus necator | - |
3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | Cutaneotrichosporon cutaneum | - |
3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | Sporotrichum pulverulentum | - |
3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | Pseudomonas sp. B13 | - |
3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | Cupriavidus necator JMP 134-1 | - |
3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | Pseudomonas sp. PKO1 | - |
3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | Cupriavidus necator JMP 134(pJP4) | - |
3-oxoadipate + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
JMP 134 | - |
Cupriavidus necator | - |
JMP 134(pJP4) | - |
Cupriavidus necator JMP 134(pJP4) | - |
JMP 134(pJP4) | - |
Cupriavidus necator JMP 134-1 | - |
JMP 134 | - |
Cutaneotrichosporon cutaneum | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. B13 | - |
- |
- |
Pseudomonas sp. PKO1 | - |
- |
- |
Sporotrichum pulverulentum | - |
lignin-degrading white-rot fungus | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
- |
Cupriavidus necator |
- |
Cutaneotrichosporon cutaneum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
600 | - |
- |
Cupriavidus necator |
Storage Stability | Organism |
---|---|
-23°C, purified enzyme in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA after freezing, storage overnight and rethawing 35% loss of activity | Cupriavidus necator |
4°C, purified enzyme can be stored in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA with slight loss of activity, 36% in 3 weeks | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloromaleylacetate + NADPH | reductive dehalogenation | Cupriavidus necator | 2-maleylacetate + Cl- + NADP+ | - |
? | |
2-chloromaleylacetate + NADPH | reductive dehalogenation | Cupriavidus necator JMP 134-1 | 2-maleylacetate + Cl- + NADP+ | - |
? | |
2-chloromaleylacetate + NADPH | reductive dehalogenation | Cupriavidus necator JMP 134(pJP4) | 2-maleylacetate + Cl- + NADP+ | - |
? | |
2-maleylacetate + NAD(P)H | - |
Pseudomonas sp. | 3-oxoadipate + NAD(P)+ | - |
ir | |
2-maleylacetate + NAD(P)H | - |
Cupriavidus necator | 3-oxoadipate + NAD(P)+ | - |
ir | |
2-maleylacetate + NAD(P)H | - |
Cutaneotrichosporon cutaneum | 3-oxoadipate + NAD(P)+ | - |
ir | |
2-maleylacetate + NAD(P)H | - |
Sporotrichum pulverulentum | 3-oxoadipate + NAD(P)+ | - |
ir | |
2-maleylacetate + NAD(P)H | - |
Pseudomonas sp. B13 | 3-oxoadipate + NAD(P)+ | - |
ir | |
2-maleylacetate + NAD(P)H | - |
Cupriavidus necator JMP 134-1 | 3-oxoadipate + NAD(P)+ | - |
ir | |
2-maleylacetate + NAD(P)H | - |
Pseudomonas sp. PKO1 | 3-oxoadipate + NAD(P)+ | - |
ir | |
2-maleylacetate + NAD(P)H | - |
Cupriavidus necator JMP 134(pJP4) | 3-oxoadipate + NAD(P)+ | - |
ir | |
2-maleylacetate + NADPH | - |
Pseudomonas sp. | 3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | - |
Cupriavidus necator | 3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | - |
Cutaneotrichosporon cutaneum | 3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | - |
Sporotrichum pulverulentum | 3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | - |
Pseudomonas sp. B13 | 3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | - |
Cupriavidus necator JMP 134-1 | 3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | - |
Pseudomonas sp. PKO1 | 3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | - |
Cupriavidus necator JMP 134(pJP4) | 3-oxoadipate + NADP+ | - |
ir | |
cis,cis-3-hydroxymuconate + NADPH | - |
Cupriavidus necator | ? + NADP+ | - |
? | |
cis,cis-3-hydroxymuconate + NADPH | - |
Cupriavidus necator JMP 134-1 | ? + NADP+ | - |
? | |
cis,cis-3-hydroxymuconate + NADPH | - |
Cupriavidus necator JMP 134(pJP4) | ? + NADP+ | - |
? | |
trans,cis-3-hydroxymuconate + NADPH | - |
Cupriavidus necator | ? + NADP+ | - |
? | |
trans,cis-3-hydroxymuconate + NADPH | - |
Cupriavidus necator JMP 134-1 | ? + NADP+ | - |
? | |
trans,cis-3-hydroxymuconate + NADPH | - |
Cupriavidus necator JMP 134(pJP4) | ? + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Pseudomonas sp. |
dimer | 2 * 35000, SDS-PAGE | Cupriavidus necator |
dimer | 2 * 40000, SDS-PAGE | Cutaneotrichosporon cutaneum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.8 | - |
Cutaneotrichosporon cutaneum |
7.5 | - |
- |
Cupriavidus necator |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | - |
Cupriavidus necator |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas sp. | |
NADH | - |
Cutaneotrichosporon cutaneum | |
NADH | NADH is preferred to NADPH | Cupriavidus necator | |
NADPH | - |
Pseudomonas sp. | |
NADPH | - |
Cupriavidus necator | |
NADPH | - |
Cutaneotrichosporon cutaneum | |
NADPH | - |
Sporotrichum pulverulentum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
3-oxoadipate | - |
Cupriavidus necator |