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Literature summary for 1.3.1.32 extracted from

  • Seibert, V.; Stadler-Fritzsche, K.; Schloemann, M.
    Purification and characterization of maleylacetate reductase from Alcaligenes eutrophus JMP134(pJP4) (1993), J. Bacteriol., 175, 6745-6754.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
histidine histidine-HCl buffer results in considerably higher activity than corresponding bisTris-HCl or Tris-HCl buffers Cupriavidus necator

Cloned(Commentary)

Cloned (Comment) Organism
-
Pseudomonas sp.

General Stability

General Stability Organism
addition of DTT significantly stabilized enzyme Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
3-oxoadipate competitive inhibition Cupriavidus necator
Mn2+
-
Cupriavidus necator
MnSO4
-
Cupriavidus necator
p-chloromercuribenzoate
-
Cupriavidus necator
p-chloromercuribenzoate
-
Cutaneotrichosporon cutaneum
p-chloromercuribenzoate
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
NADH 2-chloromaleylacetate as substrate Cupriavidus necator
0.031
-
maleylacetate
-
Cupriavidus necator
0.031
-
2-chloromaleylacetate
-
Cupriavidus necator
0.041
-
NADH maleylacetate as substrate Cupriavidus necator
0.082
-
NADPH 2-chloromaleylacetate as substrate Cupriavidus necator
0.089
-
NADPH maleylacetate as substrate Cupriavidus necator
0.09
-
maleylacetate NADPH as cosubstrate Cupriavidus necator
0.132
-
2-chloromaleylacetate NADPH as cosubstrate Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
strain PKO1 Pseudomonas sp.
35000
-
2 * 35000, SDS-PAGE Cupriavidus necator
40000
-
2 * 40000, SDS-PAGE Cutaneotrichosporon cutaneum
56000
-
gel filtration, Superdex 200 Cupriavidus necator
72000
-
analytical gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-maleylacetate + NADPH Pseudomonas sp.
-
3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH Cupriavidus necator
-
3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH Cutaneotrichosporon cutaneum
-
3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH Sporotrichum pulverulentum
-
3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH Pseudomonas sp. B13
-
3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH Cupriavidus necator JMP 134-1
-
3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH Pseudomonas sp. PKO1
-
3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH Cupriavidus necator JMP 134(pJP4)
-
3-oxoadipate + NADP+
-
ir

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
JMP 134
-
Cupriavidus necator
-
JMP 134(pJP4)
-
Cupriavidus necator JMP 134(pJP4)
-
JMP 134(pJP4)
-
Cupriavidus necator JMP 134-1
-
JMP 134
-
Cutaneotrichosporon cutaneum
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. B13
-
-
-
Pseudomonas sp. PKO1
-
-
-
Sporotrichum pulverulentum
-
lignin-degrading white-rot fungus
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.
-
Cupriavidus necator
-
Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
600
-
-
Cupriavidus necator

Storage Stability

Storage Stability Organism
-23°C, purified enzyme in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA after freezing, storage overnight and rethawing 35% loss of activity Cupriavidus necator
4°C, purified enzyme can be stored in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA with slight loss of activity, 36% in 3 weeks Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloromaleylacetate + NADPH reductive dehalogenation Cupriavidus necator 2-maleylacetate + Cl- + NADP+
-
?
2-chloromaleylacetate + NADPH reductive dehalogenation Cupriavidus necator JMP 134-1 2-maleylacetate + Cl- + NADP+
-
?
2-chloromaleylacetate + NADPH reductive dehalogenation Cupriavidus necator JMP 134(pJP4) 2-maleylacetate + Cl- + NADP+
-
?
2-maleylacetate + NAD(P)H
-
Pseudomonas sp. 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Cupriavidus necator 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Cutaneotrichosporon cutaneum 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Sporotrichum pulverulentum 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Pseudomonas sp. B13 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Cupriavidus necator JMP 134-1 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Pseudomonas sp. PKO1 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Cupriavidus necator JMP 134(pJP4) 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NADPH
-
Pseudomonas sp. 3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH
-
Cupriavidus necator 3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH
-
Cutaneotrichosporon cutaneum 3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH
-
Sporotrichum pulverulentum 3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH
-
Pseudomonas sp. B13 3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH
-
Cupriavidus necator JMP 134-1 3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH
-
Pseudomonas sp. PKO1 3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH
-
Cupriavidus necator JMP 134(pJP4) 3-oxoadipate + NADP+
-
ir
cis,cis-3-hydroxymuconate + NADPH
-
Cupriavidus necator ? + NADP+
-
?
cis,cis-3-hydroxymuconate + NADPH
-
Cupriavidus necator JMP 134-1 ? + NADP+
-
?
cis,cis-3-hydroxymuconate + NADPH
-
Cupriavidus necator JMP 134(pJP4) ? + NADP+
-
?
trans,cis-3-hydroxymuconate + NADPH
-
Cupriavidus necator ? + NADP+
-
?
trans,cis-3-hydroxymuconate + NADPH
-
Cupriavidus necator JMP 134-1 ? + NADP+
-
?
trans,cis-3-hydroxymuconate + NADPH
-
Cupriavidus necator JMP 134(pJP4) ? + NADP+
-
?

Subunits

Subunits Comment Organism
dimer
-
Pseudomonas sp.
dimer 2 * 35000, SDS-PAGE Cupriavidus necator
dimer 2 * 40000, SDS-PAGE Cutaneotrichosporon cutaneum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.8
-
Cutaneotrichosporon cutaneum
7.5
-
-
Cupriavidus necator

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5
-
Cupriavidus necator

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5
-
Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas sp.
NADH
-
Cutaneotrichosporon cutaneum
NADH NADH is preferred to NADPH Cupriavidus necator
NADPH
-
Pseudomonas sp.
NADPH
-
Cupriavidus necator
NADPH
-
Cutaneotrichosporon cutaneum
NADPH
-
Sporotrichum pulverulentum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8
-
3-oxoadipate
-
Cupriavidus necator