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Literature summary for 1.3.1.32 extracted from

  • Blasco, R.; Ramos, J.L.; Wittich, R.M.
    Pseudomonas aeruginosa strain RW41 mineralizes 4-chlorobenzenesulfonate, the major polar by-product from DDT manufacturing (2008), Environ. Microbiol., 10, 1591-1600.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme induction by 4-chlorobenzenesulfonic acid Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-maleylacetate + NAD(P)H + H+ Pseudomonas aeruginosa
-
3-oxoadipate + NAD(P)+
-
r
2-maleylacetate + NAD(P)H + H+ Pseudomonas aeruginosa RW41
-
3-oxoadipate + NAD(P)+
-
r
additional information Pseudomonas aeruginosa the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview ?
-
?
additional information Pseudomonas aeruginosa RW41 the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa O87612
-
-
Pseudomonas aeruginosa RW41 O87612
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by anion exchange chromatography Pseudomonas aeruginosa

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme is only present when cells grow on 4-chlorobenzenesulfonic acid Pseudomonas aeruginosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0034
-
substrate 2-maleylacetate, crude enzyme extract Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-maleylacetate + NAD(P)H + H+
-
Pseudomonas aeruginosa 3-oxoadipate + NAD(P)+
-
r
2-maleylacetate + NAD(P)H + H+ i.e. 3-hydroxymuconate Pseudomonas aeruginosa 3-oxoadipate + NAD(P)+
-
r
2-maleylacetate + NAD(P)H + H+
-
Pseudomonas aeruginosa RW41 3-oxoadipate + NAD(P)+
-
r
2-maleylacetate + NAD(P)H + H+ i.e. 3-hydroxymuconate Pseudomonas aeruginosa RW41 3-oxoadipate + NAD(P)+
-
r
additional information the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview Pseudomonas aeruginosa ?
-
?
additional information the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview Pseudomonas aeruginosa RW41 ?
-
?

Synonyms

Synonyms Comment Organism
maleylacetate reductase
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
Pseudomonas aeruginosa
NAD(P)H
-
Pseudomonas aeruginosa