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Literature summary for 1.3.1.33 extracted from
- Covalent labelling of the NADPH: protochlorophyllide oxidoreductase from etioplast membranes with [3H]N-phenylmaleimide (1981), Biochem. J., 195, 93-101.
General Stability
| General Stability | Organism |
|---|---|
| active enzyme is protected by NADPH, protochlorophyllide and glycerol | Phaseolus vulgaris |
| active enzyme is protected by NADPH, protochlorophyllide and glycerol | Hordeum vulgare |
| active enzyme is protected by NADPH, protochlorophyllide and glycerol | Avena sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | - |
Avena sativa |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| etioplast | - |
Phaseolus vulgaris | 9513 | - |
| etioplast | - |
Hordeum vulgare | 9513 | - |
| etioplast | - |
Avena sativa | 9513 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
- |
Avena sativa |
| 34000 | - |
2 related peptides of 34000/35000 | Phaseolus vulgaris |
| 35000 | - |
- |
Hordeum vulgare |
| 35000 | - |
2 related peptides of 34000/35000 | Phaseolus vulgaris |
| 35000 | - |
SDS-PAGE shows 2 closely related peptides of 35000 and 37000 | Avena sativa |
| 37000 | - |
- |
Hordeum vulgare |
| 37000 | - |
gel filtration, doublet of 2 peptides of 35000 and 37000 co-purified | Avena sativa |
| 38000 | - |
- |
Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protochlorophyllide + NADPH | Phaseolus vulgaris | - |
chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | Hordeum vulgare | - |
chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | Avena sativa | - |
chlorophyllide + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Avena sativa | - |
oat | - |
| Avena sativa | - |
L., var. Pennal | - |
| Hordeum vulgare | - |
barley | - |
| Hordeum vulgare | - |
L., cultivar Proctor | - |
| Phaseolus vulgaris | - |
runner bean, L., cultivar Streamline | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protochlorophyllide + NADPH | - |
Phaseolus vulgaris | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | - |
Hordeum vulgare | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | - |
Avena sativa | chlorophyllide + NADP+ | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | no reaction with NADH | Phaseolus vulgaris | |
| NADPH | no reaction with NADH | Hordeum vulgare | |
| NADPH | no reaction with NADH | Avena sativa | |
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Release 2023.1 (January 2023)
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