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Literature summary for 1.3.1.33 extracted from

  • Haeuser, I.; Dehesh, K.; Apel, K.
    The proteolytic degradation in vitro of the NADPH-protochlorophyllide oxidoreductase of barley (Hordeum vulgare L.) (1984), Arch. Biochem. Biophys., 228, 577-586.
    View publication on PubMed

General Stability

General Stability Organism
protochlorophyllide and NADPH enhances the stability of the enzyme Hordeum vulgare
rapid breakdown of the enzyme protein in vitro, caused by a membrane-bound proteolytic activity Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
PMSF
-
Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid plastid membrane Hordeum vulgare 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
-
Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protochlorophyllide + NADPH Hordeum vulgare
-
chlorophyllide + NADP+
-
?
protochlorophyllide + NADPH Avena sativa
-
chlorophyllide + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Avena sativa
-
oat
-
Hordeum vulgare
-
barley
-
Hordeum vulgare
-
L cv. carina
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Hordeum vulgare
-
leaf
-
Avena sativa
-
seedling
-
Hordeum vulgare
-
seedling
-
Avena sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protochlorophyllide + NADPH
-
Hordeum vulgare chlorophyllide + NADP+
-
?
protochlorophyllide + NADPH
-
Avena sativa chlorophyllide + NADP+
-
?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 linear drop of the relative concentration of the enzyme protein up to 30°C Hordeum vulgare

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5
-
Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
NADPH no reaction with NADH Hordeum vulgare
NADPH no reaction with NADH Avena sativa
NADPH stringent requirement Hordeum vulgare