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Literature summary for 1.3.1.33 extracted from
- Pigment-free NADPH:protochlorophyllide oxidoreductase from Avena sativa L: purification and substrate specificity (1999), Eur. J. Biochem., 265, 862-874.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Hordeum vulgare |
- |
Pisum sativum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| etioplast | - |
Hordeum vulgare | 9513 | - |
| etioplast | - |
Pisum sativum | 9513 | - |
| etioplast | - |
Arabidopsis thaliana | 9513 | - |
| etioplast | - |
Avena sativa | 9513 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity | Hordeum vulgare |  |
| Mg2+ | removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity | Avena sativa | |
| Zn2+ | removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity | Hordeum vulgare | |
| Zn2+ | removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity | Avena sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
2 related peptides of 34000/36000 | Avena sativa |
| 36000 | - |
2 related peptides of 36000/38000 | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protochlorophyllide + NADPH | Hordeum vulgare | - |
chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | Pisum sativum | - |
chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | Arabidopsis thaliana | - |
chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | Avena sativa | key enzyme for light-dependent chlorophyll biosynthesis | chlorophyllide + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Avena sativa | - |
L.cv. Pirol, var. Irlbad | - |
| Hordeum vulgare | - |
barley | - |
| Pisum sativum | - |
pea | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Hordeum vulgare | - |
| seedling | - |
Pisum sativum | - |
| seedling | - |
Arabidopsis thaliana | - |
| seedling | - |
Avena sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Hordeum vulgare |
| additional information | - |
1.49 chlorophyllide synthesis/flash per mg protein | Avena sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protochlorophyllide + NADPH | - |
Hordeum vulgare | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | - |
Pisum sativum | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | - |
Arabidopsis thaliana | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | - |
Avena sativa | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | key enzyme for light-dependent chlorophyll biosynthesis | Avena sativa | chlorophyllide + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| POR A | - |
Hordeum vulgare |
| POR A | - |
Arabidopsis thaliana |
| POR B | - |
Hordeum vulgare |
| POR B | - |
Arabidopsis thaliana |
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