General Stability | Organism |
---|---|
rapid loss of activity during attempts to purify | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stable enzyme assay system by mixing recombinant enzyme components L-protein and NB-protein under anaerobic conditiions | Rhodobacter capsulatus | |
0.0106 | - |
protochlorophyllide | pH 7.4, 34°C | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
36046 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
48671 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
52000 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
57191 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
60000 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
both activities of the components protein L and proteins NB are rapidly lost during purification procedures, such as affinity chromatography with S-protein agarose. Attempts to purify the active components have so far been unsuccessful | Rhodobacter capsulatus |
Storage Stability | Organism |
---|---|
enzyme in crude extracts stable for more than 6 months when maintained anaerobically at 4°C, with no significant loss of activity | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protochlorophyllide + NADPH | - |
Rhodobacter capsulatus | chlorophyllide + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
More | recombinant enzyme component L-protein forms a dimer, recombinant components NB-protein form a heterotetramer, gel filtration | Rhodobacter capsulatus |
Synonyms | Comment | Organism |
---|---|---|
DPOR | - |
Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Rhodobacter capsulatus |