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Literature summary for 1.3.1.33 extracted from

  • Broecker, M.J.; Virus, S.; Ganskow, S.; Heathcote, P.; Heinz, D.W.; Schubert, W.D.; Jahn, D.; Moser, J.
    ATP-driven reduction by dark-operative protochlorophyllide oxidoreductase from Chlorobium tepidum mechanistically resembles nitrogenase catalysis (2008), J. Biol. Chem., 283, 10559-10567.
    View publication on PubMed
Search for more literature for 1.3.1.33

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells Chlorobaculum tepidum

Protein Variants

Protein Variants Comment Organism
C103S the BchN subunit variant shows 0.5% residual activity and is essentially inactive Chlorobaculum tepidum
C21S the BchN subunit variant shows 0.5% residual activity and is essentially inactive Chlorobaculum tepidum
C46S the BchN subunit variant shows 0.5% residual activity and is essentially inactive Chlorobaculum tepidum

Inhibitors

Inhibitors Comment Organism Structure
cyanide
-
 Chlorobaculum tepidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0061
-
 protochlorophyllide in 100 mM HEPES-NaOH (pH 7.5), 2 mM ATP, 5 mM MgCl2 Chlorobaculum tepidum
0.0527
-
 Dithionite in 100 mM HEPES-NaOH (pH 7.5), 2 mM ATP, 5 mM MgCl2 Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protochlorophyllide + reduced ferredoxin Chlorobaculum tepidum ferredoxin is the natural electron donor chlorophyllide + oxidized ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum
-
 strain ATCC 49652
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Chlorobaculum tepidum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00315
-
 in 100 mM HEPES-NaOH (pH 7.5), 2 mM ATP, 5 mM MgCl2 Chlorobaculum tepidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protochlorophyllide + dithionite
-
 Chlorobaculum tepidum chlorophyllide + SO2
-
 ?
protochlorophyllide + dithiothreitol
-
 Chlorobaculum tepidum chlorophyllide + oxidized dithiothreitol
-
 ?
protochlorophyllide + reduced ferredoxin ferredoxin is the natural electron donor Chlorobaculum tepidum chlorophyllide + oxidized ferredoxin
-
 ?

Synonyms

Synonyms Comment Organism
dark-operative protochlorophyllide oxidoreductase
-
 Chlorobaculum tepidum
DPOR
-
 Chlorobaculum tepidum
light-independent (dark) Pchlide oxidoreductase
-
 Chlorobaculum tepidum

Cofactor

Cofactor Comment Organism Structure
4Fe-4S center
-
 Chlorobaculum tepidum
ATP
-
 Chlorobaculum tepidum