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Literature summary for 1.3.1.33 extracted from

  • Archipowa, N.; Kutta, R.J.; Heyes, D.J.; Scrutton, N.S.
    Stepwise hydridet transfer in a biological system insights into the reaction mechanism of the light-dependent protochlorophyllide oxidoreductase (2018), Angew. Chem. Int. Ed. Engl., 57, 2682-2686 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
subcloned into the expression vector pRSETA and recombinantly expressed in Escherichia coli SoluBL21 Thermosynechococcus vestitus

Protein Variants

Protein Variants Comment Organism
C226S the formed protochlorophyllide species in C226S must differ compared to those formed in wild-type enzyme, for example, by attachment of the hydride at C18 rather than C17 Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protochlorophyllide + NADPH + H+ Thermosynechococcus vestitus
-
chlorophyllide a + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Thermosynechococcus vestitus Q8DLC1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protochlorophyllide + NADPH + H+
-
Thermosynechococcus vestitus chlorophyllide a + NADP+
-
?
protochlorophyllide + NADPH + H+ stepwise hydride transfer. The enzyme catalyzes the stereospecific trans addition of a hydride anion and a proton across the C17-C18 double bond of protochlorophyllide Thermosynechococcus vestitus chlorophyllide a + NADP+
-
?

Synonyms

Synonyms Comment Organism
Light-dependent protochlorophyllide oxidoreductase
-
Thermosynechococcus vestitus
POR
-
Thermosynechococcus vestitus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Thermosynechococcus vestitus