BRENDA - Enzyme Database
show all sequences of 1.3.1.37

Studies on the metabolism of unsaturated fatty acids. II. Separation and general properties of reduced nicotinamide adenine dinucleotide phosphate dependent cis-2-enoyl-coenzyme A reductase from Escherichia coli K-12

Mizugaki, M.; Unuma, T.; Yamanaka, H.; Chem. Pharm. Bull. 27, 2334-2337 (1979)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetic acid
1 mM, 34% inhibition
Escherichia coli
p-hydroxymercuribenzoate
1 mM, 77% inhibition
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
cis-2-octenoyl-CoA
-
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0005
-
-
Escherichia coli
Storage Stability
Storage Stability
Organism
-20°C, ammonium sulfate precipitate
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cis-2-decenoyl-CoA + NADPH + H+
no reduction of cis-3-enoyl substrates
390746
Escherichia coli
decanoyl-CoA + NADP+
-
390746
Escherichia coli
?
cis-2-dodecenoyl-CoA + NADPH + H+
-
390746
Escherichia coli
dodecanoyl-CoA + NADP+
-
390746
Escherichia coli
?
cis-2-octenoyl-CoA + NADPH + H+
-
390746
Escherichia coli
octanoyl-CoA + NADP+
-
390746
Escherichia coli
-
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
no loss of activity after 30 min
Escherichia coli
55
-
10 min, inactivation
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetic acid
1 mM, 34% inhibition
Escherichia coli
p-hydroxymercuribenzoate
1 mM, 77% inhibition
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
cis-2-octenoyl-CoA
-
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0005
-
-
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, ammonium sulfate precipitate
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cis-2-decenoyl-CoA + NADPH + H+
no reduction of cis-3-enoyl substrates
390746
Escherichia coli
decanoyl-CoA + NADP+
-
390746
Escherichia coli
?
cis-2-dodecenoyl-CoA + NADPH + H+
-
390746
Escherichia coli
dodecanoyl-CoA + NADP+
-
390746
Escherichia coli
?
cis-2-octenoyl-CoA + NADPH + H+
-
390746
Escherichia coli
octanoyl-CoA + NADP+
-
390746
Escherichia coli
-
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
no loss of activity after 30 min
Escherichia coli
55
-
10 min, inactivation
Escherichia coli
Other publictions for EC 1.3.1.37
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
390745
Mizugaki
Studies on the the metabolism ...
Escherichia coli
Chem. Pharm. Bull.
30
2155-2160
1982
-
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3
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1
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1
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1
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1
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390747
Mizugaki
-
Studies on the metabolism of u ...
Escherichia coli
Chem. Pharm. Bull.
30
206-213
1982
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-
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1
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1
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1
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-
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-
-
-
-
-
-
-
-
390746
Mizugaki
Studies on the metabolism of u ...
Escherichia coli
Chem. Pharm. Bull.
27
2334-2337
1979
-
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2
1
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4
-
-
1
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1
1
3
-
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2
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-
1
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-
-
1
-
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2
-
1
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-
-
1
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-
1
1
3
-
-
-
2
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