Application | Comment | Organism |
---|---|---|
analysis | sensitive assay method measuring the fluorescence decrease of NADPH as it is converted to NADP+ during the reaction, optimized for high-throughput screening | Porphyromonas gingivalis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Porphyromonas gingivalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NADPH | pH 7.5, 25°C | Porphyromonas gingivalis | |
0.0478 | - |
crotonyl-CoA | pH 7.5, 25°C | Porphyromonas gingivalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33090 | - |
x * 33090, calculated | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | Q7MAW0 | - |
- |
Porphyromonas gingivalis W83 | Q7MAW0 | - |
- |
Storage Stability | Organism |
---|---|
-20°C, 5 mg/ml concentration, 30% glycerol stock, stable for at least 1 month | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + NADPH + H+ | - |
Porphyromonas gingivalis | butyryl-CoA + NADP+ | - |
? | |
crotonyl-CoA + NADPH + H+ | - |
Porphyromonas gingivalis W83 | butyryl-CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33090, calculated | Porphyromonas gingivalis |
Synonyms | Comment | Organism |
---|---|---|
FabK | - |
Porphyromonas gingivalis |
PG1416 | locus name | Porphyromonas gingivalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Porphyromonas gingivalis |