Cloned (Comment) | Organism |
---|---|
OPR3 expressed in functional form in Escherichia coli, cloning of OPR2 cDNA by reverse transcriptase-polymerase chain reaction, OPR2 expressed in insect cell cultures of Spodoptera frugiperda with transgenic Autographa californica baculoviruses and overexpressed in transgenic Arabidopsis thaliana | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
(9S,13S)-12-oxophytodienoic acid | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9S,13S)-12-oxo-phytodienoic acid + NADPH | Arabidopsis thaliana | OPR3 is the isoenzyme relevant for jasmonate biosynthesis | 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? | |
(9S,13S)-12-oxo-phytodienoic acid + NADPH | Capnoides sempervirens | reductase specific for the natural cis-OPDA enatiomer encoded by OPR2 | 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
(L.) Heynta wild-type ecotype Columbia(Col-0) | - |
Capnoides sempervirens | - |
- |
- |
Saccharomyces cerevisiae | - |
yeast | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
- |
Capnoides sempervirens |
OPR1, OPR2 and OPR3 | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9S,13S)-12-oxo-phytodienoic acid + NADPH | OPR3 is the isoenzyme relevant for jasmonate biosynthesis | Arabidopsis thaliana | 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? | |
(9S,13S)-12-oxo-phytodienoic acid + NADPH | reductase specific for the natural cis-OPDA enatiomer encoded by OPR2 | Capnoides sempervirens | 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OPR1 | - |
Arabidopsis thaliana |
OPR1 | - |
Capnoides sempervirens |
OPR2 | - |
Arabidopsis thaliana |
OPR3 | - |
Arabidopsis thaliana |
OYE | - |
Saccharomyces cerevisiae |