Literature summary for 1.3.1.42 extracted from

Malone, T.E.; Madson, S.E.; Wrobel, R.L.; Jeon, W.B.; Rosenberg, N.S.; Johnson, K.A.; Bingman, C.A.; Smith, D.W.; Phillips, G.N., Jr.; Markley, J.L.; Fox, B.G.
X-ray structure of Arabidopsis At2g06050, 12-oxophytodienoate reductase isoform 3 (2004), Proteins, 58, 243-245.

Cloned(Commentary)

Cloned (Comment)	Organism
cloning, and DNA sequence determination and analysis, expression of gene At2g06050, encoding isozyme OPR3, in Escherichia coli as N-terminally fusion protein fused to S-tag-His6-maltose binding protein	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
10 mg/ml purified recombinant isozyme OPR3 in 100 mM MES, pH 6.0, 100 mM NaCl, 0.3 mM TCEP, hanging drop vapour diffusion method, 0.002 ml protein solution with equal volume of well solution containing 8.75-10.0% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.275-0.35 M glycine, crystals are harvested into a cryoprotectant solution containing 12% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.4 M glycine, and 25% PEG 400, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement	Arabidopsis thaliana

Crystallization (Comment)

Organism

10 mg/ml purified recombinant isozyme OPR3 in 100 mM MES, pH 6.0, 100 mM NaCl, 0.3 mM TCEP, hanging drop vapour diffusion method, 0.002 ml protein solution with equal volume of well solution containing 8.75-10.0% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.275-0.35 M glycine, crystals are harvested into a cryoprotectant solution containing 12% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.4 M glycine, and 25% PEG 400, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement

Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	Arabidopsis thaliana	product is an intermediate in the jasmonate biosynthesis	(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	isozyme OPR3	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant isozyme OPR3 from Escherichia coli by nickel-imino-diacetic acid affinity chromatography	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	-	Arabidopsis thaliana	(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+	-	?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	product is an intermediate in the jasmonate biosynthesis	Arabidopsis thaliana	(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
OPR	-	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Arabidopsis thaliana