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Literature summary for 1.3.1.42 extracted from

  • Dong, W.; Wang, M.; Xu, F.; Quan, T.; Peng, K.; Xiao, L.; Xia, G.
    Wheat oxophytodienoate reductase gene TaOPR1 confers salinity tolerance via enhancement of abscisic acid signaling and reactive oxygen species scavenging (2013), Plant Physiol., 161, 1217-1228 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.42

Cloned(Commentary)

Cloned (Comment) Organism
gene TaOPR1, TaOPR1 maps to the short arm of the second chromosome of the B genome (2BS), DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression in Arabidopsis thaliana ecotype Col-0. The repression of AtOPR1 by the TaOPR1 transgene suggests a negative feedback regulatory effect, perhaps based on functional redundancy between TaOPR1 and AtOPR1 Triticum aestivum

Protein Variants

Protein Variants Comment Organism
additional information overexpression of TaOPR1 in wheat significantly enhances the level of salinity tolerance, while its heterologous expression in Arabidopsis thaliana alleviates root growth restriction in the presence of salinity and oxidants and raises the sensitivity to abscisic acid. In Arabidopsis, recombinant TaOPR1 promotes abscisic acid synthesis. and the abscisic acid-dependent stress-responsive pathway. Recombinant TaOPR1 partially rescues the sensitivity of the Arabidopsis thaliana aba2 mutant defective in abscisic acid synthesis to salinity, and improves the activities of reactive oxygen species scavengers and the transcription of their encoding genes while reducing malondialdehyde and reactive oxygen species levels. After exposure to 200 mM NaCl, the wild-type seedlings become very wilted while the TaOE ones remain fully turgid. The TaOE line shoots and roots are longer than those of the wild type, phenotype, overview. Inconsistent with the positive effect of TaOPR1, the double RNA interference (RNAi) knockdown mutant opr1opr2, from two homologous OPRI genes in Arabidopsis thaliana, do not increase the sensitivity to NaCl treatment, and their phenotypes are comparable to the wild-type. TaOPR1-overexpressing lines show insensitivity to H2O2 Triticum aestivum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Triticum aestivum 5737
-
additional information the enzyme lacks the OPRII-specific peroximal signal peptide at its C terminus Triticum aestivum
-
-

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
 salinity-tolerant bread wheat cultivar SR3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 Triticum aestivum (15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, about, sequence calculation Triticum aestivum

Synonyms

Synonyms Comment Organism
OPRI
-
 Triticum aestivum
oxophytodienoate reductase
-
 Triticum aestivum
TaOPR1
-
 Triticum aestivum

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Triticum aestivum
NADPH
-
 Triticum aestivum

pI Value

Organism Comment pI Value Maximum pI Value
Triticum aestivum sequence calculation
-
 6.52

Expression

Organism Comment Expression
Triticum aestivum salinity stress induces a higher level of TaOPR1 expression in the seedling roots of cv. SR3 compared to parental cultivar JN177. This induction is abolished when abscisic acid (ABA) synthesis is inhibited up

General Information

General Information Comment Organism
evolution the TaOPR1 sequence retains the conserved residues that account for the NADPH- and FMN-binding sites of the OYE family Triticum aestivum
metabolism enzyme TaOPR1 stimulated the abscisic acid-dependent stress-responsive pathway Triticum aestivum
physiological function enzyme TaOPR1 does not interact with jasmonate synthesis or the jasmonate signaling pathway. Rather than serving purely as an antioxidant, TaOPR1 acts during episodes of abiotic stress response as a signaling compound associated with the regulation of the abscisic acid-mediated signaling network. Overexpression of TaOPR1 in wheat significantly enhances the level of salinity tolerance, while its heterologous expression in Arabidopsis thaliana alleviates root growth restriction in the presence of salinity and oxidants and raises the sensitivity to abscisic acid. In Arabidopsis, recombinant TaOPR1 promotes abscisic acid synthesis. and the abscisic acid-dependent stress-responsive pathway. Recombinant TaOPR1 partially rescues the sensitivity of the Arabidopsis thaliana aba2 mutant defective in abscisic acid synthesis to salinity, and improves the activities of reactive oxygen species scavengers and the transcription of their encoding genes while reducing malondialdehyde and reactive oxygen species levels. TaOPR1 enhanced the salinity tolerance of wheat and in transgenic Arabidopsis thaliana. TaOPR1-overexpressing lines show insensitivity to H2O2 Triticum aestivum