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Literature summary for 1.3.1.42 extracted from

  • Maynard, D.; Kumar, V.; Sproi, J.; Dietz, K.J.
    12-Oxophytodienoic acid reductase 3 (OPR3) functions as NADPH-dependent alpha,beta-ketoalkene reductase in detoxification and monodehydroascorbate reductase in redox homeostasis (2020), Plant Cell Physiol., 61, 584-595 .
    View publication on PubMed
Search for more literature for 1.3.1.42

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0283
-
 (15Z)-12-oxophyto-10,15-dienoate pH 7.2, temperature not specified in the publication Arabidopsis thaliana
0.0385
-
 Methyl vinyl ketone pH 7.2, temperature not specified in the publication Arabidopsis thaliana
1.967
-
 2-methylcyclopent-2-en-1-one pH 7.2, temperature not specified in the publication Arabidopsis thaliana
4.36
-
 trans-hex-2-enal pH 7.2, temperature not specified in the publication Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FUP0 cf. EC 1.6.5.4
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6
-
 substrate maleic acid, pH 7.2, temperature not specified in the publication Arabidopsis thaliana
0.6
-
 substrate trans-hex-2-enal, pH 7.2, temperature not specified in the publication Arabidopsis thaliana
2
-
 substrate (15Z)-12-oxophyto-10,15-dienoate, pH 7.2, temperature not specified in the publication Arabidopsis thaliana
2.5
-
 substrate 2-methylcyclopent-2-en-1-one, pH 7.2, temperature not specified in the publication Arabidopsis thaliana
13.5
-
 substrate methyl vinyl ketone, pH 7.2, temperature not specified in the publication Arabidopsis thaliana
22.8
-
 substrate N-ethyl-maleimide, pH 7.2, temperature not specified in the publication Arabidopsis thaliana
26.9
-
 substrate 1,4-benzoquinone, pH 7.2, temperature not specified in the publication Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
-
 Arabidopsis thaliana 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1,4-benzoquinone + NADPH + H+
-
 Arabidopsis thaliana ? + NADP+
-
 ?
2-methylcyclopent-2-en-1-one + NADPH + H+
-
 Arabidopsis thaliana (2S)-2-methylcyclopentanone + NAD+
-
 ?
maleic acid + NADPH + H+
-
 Arabidopsis thaliana ? + NADP+
-
 ?
methyl vinyl ketone + NADPH + H+
-
 Arabidopsis thaliana hexanal + NADP+
-
 ?
N-ethyl-maleimide + NADPH + H+
-
 Arabidopsis thaliana ? + NAD+
-
 ?
trans-hex-2-enal + NADPH + H+
-
 Arabidopsis thaliana hexanal + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE, x * 42690, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
12-oxophytodienoate reductase 3
-
 Arabidopsis thaliana
OPR3
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FMN absorption maxima at 280, 374 and 467 nm Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function OPR3 is a bifunctional enzyme with NADPH-dependent alpha,beta-ketoalkene double-bond reductase and monodehydroascorbate reductase activities. OPR3 mutants show a slightly less-reduced ascorbate pool in leaves in line with the MDHAR activity of OPR3 in vitro. OPR3 catalyzes the reduction of a wide spectrum of electrophilic species that share a reactivity toward the glutathione and ascorbate. 12-oxophytodienoic acid reacts with ascorbate to form an ascorbate-12-oxophytodienoic acid adduct, but in addition OPR3 has the ability to regenerate ascorbate from monodehydroascorbate Arabidopsis thaliana