BRENDA - Enzyme Database
show all sequences of 1.3.1.44

Kinetic evidence for two separate trans-2-enoyl CoA reductases in rat hepatic microsomes: NADPH-specific short chain- and NAD(P)H-dependent long chain-reductase

Prasad, M.R.; Nagi, M.N.; Cook, L.; Cinti, D.L.; Biochem. Biophys. Res. Commun. 113, 659-665 (1983)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.005
-
substrate trans-2-octenoyl-CoA with NADH
Rattus norvegicus
0.02
-
substrate trans-2-hexadecenoyl-CoA with NADH
Rattus norvegicus
0.027
-
substrate trans-2-hexadecenoyl-CoA with NADPH
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
long-chain substrates preferred
390755
Rattus norvegicus
?
-
-
-
?
trans-2-hexadecenoyl-CoA + NADH
-
390755
Rattus norvegicus
hexadecanoyl-CoA + NAD+
-
-
-
?
trans-2-octenoyl-CoA + NADH
-
390755
Rattus norvegicus
octanoyl-CoA + NAD+
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
preferred cofactor with substrates of higher chain length
Rattus norvegicus
NADPH
-
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
preferred cofactor with substrates of higher chain length
Rattus norvegicus
NADPH
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.005
-
substrate trans-2-octenoyl-CoA with NADH
Rattus norvegicus
0.02
-
substrate trans-2-hexadecenoyl-CoA with NADH
Rattus norvegicus
0.027
-
substrate trans-2-hexadecenoyl-CoA with NADPH
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
long-chain substrates preferred
390755
Rattus norvegicus
?
-
-
-
?
trans-2-hexadecenoyl-CoA + NADH
-
390755
Rattus norvegicus
hexadecanoyl-CoA + NAD+
-
-
-
?
trans-2-octenoyl-CoA + NADH
-
390755
Rattus norvegicus
octanoyl-CoA + NAD+
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Other publictions for EC 1.3.1.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Schadeweg
Increasing n-butanol productio ...
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1
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1
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1
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-
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-
-
-
-
-
-
-
-
-
724240
Hu
Structures of trans-2-enoyl-Co ...
Treponema denticola, Clostridium acetobutylicum, Treponema denticola ATCC 35405
Biochem. J.
449
79-89
2013
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-
2
4
10
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-
10
-
-
3
2
-
17
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-
2
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-
-
-
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10
4
8
2
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-
10
2
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-
4
-
-
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-
-
2
4
4
10
-
-
-
-
10
-
-
3
2
-
-
-
2
-
-
-
-
10
4
2
-
-
10
2
-
-
-
-
-
-
-
10
10
724351
Bond-Watts
Biochemical and structural cha ...
Treponema denticola, Treponema denticola ATCC 35405
Biochemistry
51
6827-6837
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1
2
7
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3
36
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-
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-
5
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-
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11
-
4
-
-
-
36
-
-
-
2
14
-
-
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-
1
2
2
7
-
-
3
14
36
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
36
-
-
-
-
-
-
-
-
38
38
723850
Lee
Crystallization and preliminar ...
Pseudomonas aeruginosa
Acta Crystallogr. Sect. F
67
214-216
2011
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-
1
1
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1
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1
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1
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1
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2
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1
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1
1
1
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1
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1
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1
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711734
Mattheus
The kalimantacin/batumin biosy ...
Pseudomonas fluorescens, Pseudomonas fluorescens BCCM_ID9359
Chem. Biol.
17
1067-1071
2010
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-
1
-
1
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-
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-
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4
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3
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4
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1
4
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1
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-
2
2
-
-
-
686722
Tucci
A novel prokaryotic trans-2-en ...
Treponema denticola
FEBS Lett.
581
1561-1566
2007
-
-
1
-
-
-
2
1
-
-
1
-
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1
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1
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1
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2
1
2
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1
1
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1
1
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2
1
1
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1
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1
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1
-
2
1
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-
-
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656287
Hoffmeister
Mitochondrial trans-2-enoyl-Co ...
Euglena gracilis
J. Biol. Chem.
280
4329-4338
2005
-
-
1
-
-
-
-
8
1
-
1
2
-
16
-
-
2
-
-
1
8
-
5
2
2
1
-
-
-
1
-
-
4
-
-
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-
-
1
4
-
-
-
-
-
-
8
1
-
1
2
-
-
-
2
-
1
8
-
5
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657270
Poletto
Selection of an Escherichia co ...
Mycobacterium tuberculosis
Protein Expr. Purif.
34
118-125
2004
-
1
1
-
4
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
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-
3
-
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1
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1
1
1
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4
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1
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390806
Inui
Purification and some properti ...
Euglena gracilis
J. Biochem.
100
995-1000
1986
-
-
-
-
-
-
7
-
1
-
3
1
-
1
-
-
1
1
-
-
1
-
2
1
-
1
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
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-
7
-
-
1
-
3
1
-
-
-
1
-
-
1
-
2
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
390810
Inui
Fatty acid synthesis in mitoch ...
Euglena gracilis
Eur. J. Biochem.
142
121-126
1984
-
-
-
-
-
-
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-
1
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1
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5
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2
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2
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1
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5
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-
-
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-
390754
Nagi
Biochemical properties of shor ...
Rattus norvegicus
Arch. Biochem. Biophys.
226
50-64
1983
-
-
-
-
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-
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1
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1
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1
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-
-
2
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-
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-
-
-
-
-
-
-
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-
390755
Prasad
Kinetic evidence for two separ ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
113
659-665
1983
-
-
-
-
-
-
-
-
1
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-
1
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3
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3
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1
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2
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-
2
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1
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3
-
3
-
1
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-
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390807
Shimakata
Purification and characterizat ...
Mycolicibacterium smegmatis
J. Biochem.
89
1075-1080
1981
-
-
-
-
-
-
4
5
-
-
1
1
-
1
-
-
1
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1
1
9
1
1
-
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1
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1
1
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-
1
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4
1
5
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1
1
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1
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1
1
9
1
-
-
-
-
1
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-
-
-
-
-
-
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390808
Shimakata
Involvement of one of two enoy ...
Mycolicibacterium smegmatis
J. Biochem.
88
1051-1058
1980
-
-
-
-
-
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1
1
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1
1
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1
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1
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1
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5
-
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1
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1
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1
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1
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1
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1
1
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1
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1
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5
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1
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286051
Miller
Enzymology of butyrate formati ...
Butyrivibrio fibrisolvens
J. Bacteriol.
138
99-104
1979
-
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1
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1
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1
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1
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3
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3
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1
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