Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | only after preincubation with cation | Euglena gracilis | |
KCN | protection by FAD | Euglena gracilis | |
Mn2+ | only after preincubation with cation | Euglena gracilis | |
N-ethylmaleimide | protection by FAD | Euglena gracilis | |
Ni2+ | only after preincubation with cation | Euglena gracilis | |
p-chloromercuribenzoate | protection by FAD | Euglena gracilis | |
Zn2+ | only after preincubation with cation | Euglena gracilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Euglena gracilis | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
1 * 15000 + 1 * 25000, SDS-PAGE | Euglena gracilis |
25000 | - |
1 * 15000 + 1 * 25000, SDS-PAGE | Euglena gracilis |
39000 | - |
gel filtration | Euglena gracilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + NAD+ | Euglena gracilis | contributes to fatty acid synthesis beta-oxidation in mitochondria | trans-2,3-didehydroacyl-CoA + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Euglena gracilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acyl-CoA + NAD+ = trans-didehydroacyl-CoA + NADH + H+ | short chain-length specific | Euglena gracilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
- |
Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + NAD+ | contributes to fatty acid synthesis beta-oxidation in mitochondria | Euglena gracilis | trans-2,3-didehydroacyl-CoA + NADH | - |
? | |
crotonyl-CoA + NADH | negative cooperativity in the reaction with substrate | Euglena gracilis | butyryl-CoA + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 15000 + 1 * 25000, SDS-PAGE | Euglena gracilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
at pH 6.2 | Euglena gracilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Euglena gracilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 7 | less than 50% of maximal activity above pH 7.0 and below pH 5.2 | Euglena gracilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | FAD, riboflavin and FMN increase activity | Euglena gracilis | |
NADH | sole electron donor | Euglena gracilis |