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Literature summary for 1.3.1.44 extracted from

  • Hoffmeister, M.; Piotrowski, M.; Nowitzki, U.; Martin, W.
    Mitochondrial trans-2-enoyl-CoA reductase of wax ester fermentation from Euglena gracilis defines a new family of enzymes involved in lipid synthesis (2005), J. Biol. Chem., 280, 4329-4338.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
phage library screening, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression as His-tagged enzyme in Escherichia coli Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
crotonyl-CoA pH 6.2, 30°C Euglena gracilis
0.068
-
crotonyl-CoA recombinant enzyme, pH 6.2, 30°C, with NADH as cofactor Euglena gracilis
0.091
-
trans-2-hexenoyl-CoA pH 6.2, 30°C Euglena gracilis
0.091
-
trans-2-hexenoyl-CoA recombinant enzyme, pH 6.2, 30°C, with NADH as cofactor Euglena gracilis
0.109
-
Biochanin A recombinant enzyme, pH 6.2, 30°C, with crotonyl-CoA as substrate Euglena gracilis
0.109
-
Biochanin A cosubstrate crotonyl-CoA, pH 6.2, 30°C Euglena gracilis
0.119
-
NADPH recombinant enzyme, pH 6.2, 30°C, with crotonyl-CoA as substrate Euglena gracilis
0.119
-
NADPH cosubstrate crotonyl-CoA, pH 6.2, 30°C Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Euglena gracilis 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Euglena gracilis enzyme is involved in malonyl-CoA independent lipid synthesis and wax ester fermentation ?
-
?
trans-didehydroacyl-CoA + NAD(P)H Euglena gracilis i.e. enoyl-CoA acyl-CoA + NAD(P)+
-
?

Organism

Organism UniProt Comment Textmining
Euglena gracilis Q5EU90
-
-
Euglena gracilis Q5EU90 mitochondrial precursor
-

Purification (Commentary)

Purification (Comment) Organism
-
Euglena gracilis
native enzyme 1687fold to homogeneity in several steps, recombinant His-tagged enzyme from Escherichia coli by affinity chromatography Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme is expressed under aerobic and anaerobic conditions Euglena gracilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.078
-
mitochondria, anaerobic conditions, substrates crotonyl-CoA and NADH Euglena gracilis
0.086
-
mitochondria, aerobic conditions, substrates crotonyl-CoA and NADH Euglena gracilis
0.37
-
purified recombinant enzyme, substrates trans-hexenoyl-CoA and NADPH Euglena gracilis
0.66
-
purified recombinant enzyme, substrates crotonyl-CoA and NADPH Euglena gracilis
1.45
-
purified recombinant enzyme, substrates trans-hexenoyl-CoA and NADH Euglena gracilis
1.65
-
purified recombinant enzyme, substrates crotonyl-CoA and NADH Euglena gracilis
3.88
-
pH 6.2, 30°C Euglena gracilis
3.88
-
purified native enzyme, substrate crotonyl-CoA and NADH Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + NAD(P)H
-
Euglena gracilis butyryl-CoA + NAD(P)+
-
?
crotonyl-CoA + NADH + H+
-
Euglena gracilis butyryl-CoA + NAD+
-
r
additional information enzyme is involved in malonyl-CoA independent lipid synthesis and wax ester fermentation Euglena gracilis ?
-
?
trans-2-hexenoyl-CoA + NAD(P)H
-
Euglena gracilis hexanoyl-CoA + NAD(P)+
-
?
trans-didehydroacyl-CoA + NAD(P)H i.e. enoyl-CoA Euglena gracilis acyl-CoA + NAD(P)+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 44000, SDS-PAGE Euglena gracilis
monomer 1 x 44000, SDS-PAGE Euglena gracilis

Synonyms

Synonyms Comment Organism
TER
-
Euglena gracilis
trans-2-enoyl-CoA reductase
-
Euglena gracilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
assay at Euglena gracilis

Cofactor

Cofactor Comment Organism Structure
NADH preferred, 2-3fold higher activity compared to NADPH Euglena gracilis
NADH about two-to threefold higher specific activites with NADH over NADPH Euglena gracilis
NADPH 2-3fold lower activity compared to the preferred NADH Euglena gracilis
NADPH about two-to threefold higher specific activites with NADH over NADPH Euglena gracilis