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Literature summary for 1.3.1.45 extracted from

  • Kim, S.G.; Kim, S.T.; Wang, Y.; Kim, S.K.; Lee, C.H.; Kim, K.K.; Kim, J.K.; Lee, S.Y.; Kang, K.Y.
    Overexpression of rice isoflavone reductase-like gene (OsIRL) confers tolerance to reactive oxygen species (2009), Physiol. Plant., 138, 1-9.
    View publication on PubMed
Search for more literature for 1.3.1.45

Cloned(Commentary)

Cloned (Comment) Organism
generating of transgenic rice lines overexpressing the OsIRL gene under an abscisic acid inducible promoter, the OsIRL transgenic rice line activated by abscisic acid treatment is tolerant against methyl viologen and glucose/glucose oxidase-induced stress in rice leaves and suspension-cultured cells, cloning of recombinant enzyme as six-His tagged OsIRL protein for antibody production Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-hydroxyformononetin + NADPH + H+ Oryza sativa key enzyme in isoflavonoid phytoalexin biosynthesis vestitone + NADP+
-
 ?
additional information Oryza sativa involvement of OsIRL in homeostasis of reactive oxygen species, products of OsIRL are positive regulators to suppress cell death under oxidative stress ?
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 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-
Oryza sativa Q8VYH7 L. cv. Jinheung
-

Purification (Commentary)

Purification (Comment) Organism
as six-His tagged OsIRL protein, the generated recombinant OsIRL protein is used for antibody preparation Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
callus
-
 Oryza sativa
-
cell suspension culture
-
 Oryza sativa
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root
-
 Oryza sativa
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seedling
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-hydroxyformononetin + NADPH + H+ key enzyme in isoflavonoid phytoalexin biosynthesis Oryza sativa vestitone + NADP+
-
 ?
additional information involvement of OsIRL in homeostasis of reactive oxygen species, products of OsIRL are positive regulators to suppress cell death under oxidative stress Oryza sativa ?
-
 ?

Synonyms

Synonyms Comment Organism
IFR
-
 Oryza sativa
isoflavone reductase
-
 Oryza sativa
OsIRL
-
 Oryza sativa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Oryza sativa

Expression

Organism Comment Expression
Oryza sativa After embedding of seeds in agar containing 2',7',-dichlorofluorescin, the H2O2 is highly stimulated in the elongation region compared with the root apex. The OsIRL mRNA expression level in 3-day-old roots is weakly expressed in the apex region, it shows an increment accompanying the increase in H2O2 concentration, results indicate that the expression level and localization of OsIRL is associated with H2O2 accumulation. down
Oryza sativa OsIRL gene and protein are downregulated in young seedling roots treated with diphenyleneiodonium, known quencher of reactive oxygen species, but to a lesser extent when compared with glutathione treatment in roots down
Oryza sativa OsIRL gene and protein are downregulated in young seedling roots treated with reduced glutathione, a known quencher of reactive oxygen species, in roots of 3 day-old young seedlings grown on glutathione, reactive oxygen species levels are significantly decreased by approximately 50% in the presence of 1 mM glutathione compared with untreated controls down
Oryza sativa OsIRL gene and protein are shown to be downregulated in young seedling roots treated with reduced glutathione and diphenyleneiodonium down
Oryza sativa the expression of OsIRL is slightly increased after treatment 0.02 mM abscisic acid in wild-type calli, suggesting a possible reason for glucose/glucose oxidase stress resistance up
Oryza sativa the OsIRL transcript level in rice suspension-cultured cells is induced by FeCl3 within 4 h after treatment, but down-regulated when co-treated with glutathione up
Oryza sativa the OsIRL transcript level in rice suspension-cultured cells is induced by glucose/glucose oxidase within 4 h after treatment, but down-regulated when co-treated with glutathione up
Oryza sativa the OsIRL transcript level in rice suspension-cultured cells is induced by methyl viologen within 4 h after treatment, but down-regulated when co-treated with glutathione up
Oryza sativa the OsIRL transcript level in rice suspension-cultured cells is induced by the oxidant H2O2, but down-regulated when co-treated with glutathione up
Oryza sativa OsIRL transcript level in rice suspension-cultured cells is found to be by hydrogen peroxide, ferric chloride, methyl viologen and glucose/glucose oxidase up

General Information

General Information Comment Organism
malfunction transgenic rice lines overexpressing the OsIRL gene under an abscisic acid inducible promoter are tolerant against methyl viologen (MV) and glucose/glucose oxidase-induced stress in rice leave and suspension-cultured cells Oryza sativa