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Literature summary for 1.3.1.48 extracted from

  • Westbrook, C.; Jarabak, J.
    15-Ketoprostaglandin delta13 reductase from human placenta: purification, kinetics, and inhibitor binding (1978), Arch. Biochem. Biophys., 185, 429-442.
    View publication on PubMed
Search for more literature for 1.3.1.48

Inhibitors

Inhibitors Comment Organism Structure
13,14-dihydro-15-prostaglandin E2 competitive product inhibition Homo sapiens
Cibacron blue 3 G-A
-
 Homo sapiens
NADH uncompetitive inhibition at high concentrations Homo sapiens
NADPH
-
 Homo sapiens
p-chloromercuribenzoate 0.0001 mM, 85% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
 15-ketoprostaglandin E1
-
 Homo sapiens
0.0075
-
 15-ketoprostaglandin A1
-
 Homo sapiens
0.0108
-
 15-ketoprostaglandin F2alpha
-
 Homo sapiens
0.0112
-
 15-ketoprostaglandin B1
-
 Homo sapiens
0.0127
-
 15-ketoprostaglandin E2
-
 Homo sapiens
0.032
-
 NADH
-
 Homo sapiens
0.063
-
 reduced nicotinamide hypoxanthine dinucleotide phosphate
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Homo sapiens
68500
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.393
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol v/v, 5 mM potassium phosphate, 1 mM EDTA, pH 7.0, stable for at least 6 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H 55% of activity with 15-keto-prostaglandin E2 Homo sapiens (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
 ir
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H i.e. 15-ketoprostaglandin E2 Homo sapiens 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
 ?
15-ketoprostaglandin A1 + NAD(P)H 108% of activity with 15-keto-prostaglandin E2 Homo sapiens ?
-
 ?
15-ketoprostaglandin B1 + NAD(P)H 79% of activity with 15-keto-prostaglandin E2 Homo sapiens ?
-
 ?
15-ketoprostaglandin E1 + NADPH
-
 Homo sapiens ?
-
 ?
15-ketoprostaglandin E2 + NADH + H+ 100% activity Homo sapiens 13,14-dihydro-15-ketoprostaglandin E2 + NAD+
-
 r
15-ketoprostaglandin E2 + reduced nicotinamide hypoxanthine dinucleotide + H+ 93% activity compared to NADH Homo sapiens 13,14-dihydro-15-ketoprostaglandin E2 + nicotinamide hypoxanthine dinucleotide
-
 r
15-ketoprostaglandin F2alpha + NADPH
-
 Homo sapiens ?
-
 ?
9,11-dihydroxy-15-oxoprosta-5,13-dien-1-oic acid + NAD(P)H 56% of activity with 15-keto-prostaglandin E2 Homo sapiens 9,11-dihydroxy-15-oxoprosta-5-en-1-oic aicd + NAD(P)+
-
 ?
9,11-dihydroxy-15-oxoprosta-5,13-dien-1-oic acid + NAD(P)H i.e. 15-ketoprostaglandin F2alpha Homo sapiens 9,11-dihydroxy-15-oxoprosta-5-en-1-oic aicd + NAD(P)+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
 Homo sapiens
NADH human placenta: enzyme can use only NADH Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00001
-
 NADPH
-
 Homo sapiens
0.0000125
-
 Cibacron Blue 3G-A
-
 Homo sapiens
0.079
-
 13,14-dihydro-15-prostaglandin E2
-
 Homo sapiens