Literature summary for 1.3.1.54 extracted from

Blanche, F.; Thibaut, D.; Famechon, A.; Debussche, L.; Cameron, B.; Crouzet, J.
Precorrin-6x reductase from Pseudomonas denitrificans: purification and characterization of the enzyme and identification of the structural gene (1992), J. Bacteriol., 174, 1036-1042.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0036	-	precorrin-6X	-	Pseudomonas denitrificans (nom. rej.)
0.0235	-	NADPH	-	Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	gel filtration	Pseudomonas denitrificans (nom. rej.)
28080	-	calculation from nucleotide sequence	Pseudomonas denitrificans (nom. rej.)
31000	-	1 * 31000, SDS-PAGE	Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Precorrin-6X + NADPH	Pseudomonas denitrificans (nom. rej.)	reduction step along the pathway to corrins	Precorrin-6Y + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas denitrificans (nom. rej.)	-	recombinant strain	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas denitrificans (nom. rej.)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.127	-	-	Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Precorrin-6X + NADPH	-	Pseudomonas denitrificans (nom. rej.)	Precorrin-6Y + NADP+	-	?
Precorrin-6X + NADPH	reduction step along the pathway to corrins	Pseudomonas denitrificans (nom. rej.)	Precorrin-6Y + NADP+	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 31000, SDS-PAGE	Pseudomonas denitrificans (nom. rej.)

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Pseudomonas denitrificans (nom. rej.)

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Release 2023.1 (January 2023)