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Literature summary for 1.3.1.56 extracted from
- Characterization of active recombinant 2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase from Comamonas testosteroni B-356 and sequence of the encoding gene (bphB) (1996), Appl. Environ. Microbiol., 62, 2710-2715.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.073 | - |
2,3-dihydro-2,3,-dihydroxybiphenyl | activity measured with 1 micromol NAD+ in the reaction mixture at pH 9.5 and 37°C | Comamonas testosteroni |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 123000 | - |
native protein, gel filtration | Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD+ | Comamonas testosteroni | biphenyl degradation pathway | 2,3-dihydroxybiphenyl + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | - |
gene bphB | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 46 | - |
- |
Comamonas testosteroni |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C for months | Comamonas testosteroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD+ | - |
Comamonas testosteroni | 2,3-dihydroxybiphenyl + NADH | - |
? | |
| cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD+ | biphenyl degradation pathway | Comamonas testosteroni | 2,3-dihydroxybiphenyl + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Comamonas testosteroni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Comamonas testosteroni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
recombinant His-tagged enzyme | Comamonas testosteroni |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | essential cofactor for His-taggged enzyme | Comamonas testosteroni | |
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