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Literature summary for 1.3.1.57 extracted from

  • Haddock, J.D.; Ferry, J.G.
    Purification and properties of phloroglucinol reductase from Eubacterium oxidoreducens G-41 (1989), J. Biol. Chem., 264, 4423 - 4427.
    View publication on PubMed
Search for more literature for 1.3.1.57

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trihydroxybenzoate tested at 1.0 mM and under N2, 49% inhibition Eubacterium oxidoreducens
6-Hydroxynicotinate tested at 1.0 mM, 12% inhibition Eubacterium oxidoreducens
catechol tested at 1.0 mM, 14% inhibition Eubacterium oxidoreducens
CuCl2 tested at 0.1 mM, 100% inhibition Eubacterium oxidoreducens
diethyldicarbonate tested at 1.0 mM, 54% inhibition Eubacterium oxidoreducens
gallate tested at 1.0 mM and under N2, 24% inhibition Eubacterium oxidoreducens
hydroquinone tested at 1.0 mM and under N2, 31% inhibition Eubacterium oxidoreducens
menadione tested at 1.0 mM, 33% inhibition Eubacterium oxidoreducens
N-ethylmaleimide tested at 1.0 mM, 94% inhibition Eubacterium oxidoreducens
p-chloromercuribenzoate tested at 0.1 mM, 100% inhibition Eubacterium oxidoreducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0067
-
 NADPH at pH 6.8 Eubacterium oxidoreducens
0.027
-
 NADP+ reverse direction at pH 7.2 Eubacterium oxidoreducens
0.29
-
 dihydrophloroglucinol reverse direction at pH 7.2 Eubacterium oxidoreducens
0.56
-
 phloroglucinol at pH 7.6 Eubacterium oxidoreducens
0.6
-
 phloroglucinol at pH 7.2 Eubacterium oxidoreducens
0.8
-
 phloroglucinol at pH 6.8 Eubacterium oxidoreducens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 2 * alpha, 2 * 33000, gel electrophoresis Eubacterium oxidoreducens
78000
-
 gel filtration chromatography with native enzyme Eubacterium oxidoreducens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phloroglucinol + NADPH Eubacterium oxidoreducens pathway of anaerobic degradation of trihydroxybenzenes and metabolism of gallic acid dihydrophloroglucinol + NADP
-
 r
phloroglucinol + NADPH Eubacterium oxidoreducens G-41 pathway of anaerobic degradation of trihydroxybenzenes and metabolism of gallic acid dihydrophloroglucinol + NADP
-
 r

Organism

Organism UniProt Comment Textmining
Eubacterium oxidoreducens
-
-
-
Eubacterium oxidoreducens G-41
-
-
-

Purification (Commentary)

Purification (Comment) Organism
prepared of a cell extract by French press, DEAE-cellulose column, phenyl-Sepharose column, ultrafiltration, red agarose column, anion exchange column, purified 90.4fold with a yield of 29% Eubacterium oxidoreducens

Source Tissue

Source Tissue Comment Organism Textmining
rumen organism is a strict anaerobe Eubacterium oxidoreducens
-

Storage Stability

Storage Stability Organism
stable for several hours when kept on ice and retained full activity being frozen with 15% glycerol in liquid N2. Incubation at 30°C and pH 7.0, 82% of initial activity retained after 3.3 h Eubacterium oxidoreducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phloroglucinol + NADPH
-
 Eubacterium oxidoreducens dihydrophloroglucinol + NADP+
-
 r
phloroglucinol + NADPH
-
 Eubacterium oxidoreducens G-41 dihydrophloroglucinol + NADP+
-
 r
phloroglucinol + NADPH pathway of anaerobic degradation of trihydroxybenzenes and metabolism of gallic acid Eubacterium oxidoreducens dihydrophloroglucinol + NADP
-
 r
phloroglucinol + NADPH pathway of anaerobic degradation of trihydroxybenzenes and metabolism of gallic acid Eubacterium oxidoreducens G-41 dihydrophloroglucinol + NADP
-
 r

Subunits

Subunits Comment Organism
dimer 2 * alpha, 2 * 33000, gel electrophoresis Eubacterium oxidoreducens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Eubacterium oxidoreducens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Eubacterium oxidoreducens