Cloned (Comment) | Organism |
---|---|
sequence comparisons, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Eubacterium oxidoreducens |
sequence comparisons, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Clostridium sp. |
sequence comparisons, recombinant overexpression of His-tagged enzyme in Escherichia coli train BL21(DE3) | Desulfosporosinus orientis |
Crystallization (Comment) | Organism |
---|---|
three dimensional structures of PGRcl in the apo form and with bound NADPH are determined to 1.7 A and 1.8 A resolution, respectively. The enzyme crystallizes as a homotetramer (125 kDa), with two monomers in the asymmetric unit | Clostridium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Desulfosporosinus orientis | |
additional information | - |
additional information | Michaelis-Menten kinetics | Eubacterium oxidoreducens | |
additional information | - |
additional information | Michaelis-Menten kinetics | Clostridium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
- |
Eubacterium oxidoreducens |
125000 | - |
- |
Clostridium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phloroglucinol + NADPH + H+ | Desulfosporosinus orientis | - |
dihydrophloroglucinol + NADP+ | - |
? | |
phloroglucinol + NADPH + H+ | Eubacterium oxidoreducens | - |
dihydrophloroglucinol + NADP+ | - |
? | |
phloroglucinol + NADPH + H+ | Clostridium sp. | - |
dihydrophloroglucinol + NADP+ | - |
? | |
phloroglucinol + NADPH + H+ | Eubacterium oxidoreducens AB3007 | - |
dihydrophloroglucinol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sp. | U2C7W9 | - |
- |
Desulfosporosinus orientis | - |
- |
- |
Eubacterium oxidoreducens | P57793 | - |
- |
Eubacterium oxidoreducens AB3007 | P57793 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli train BL21(DE3) by nickel affinity chromatography | Desulfosporosinus orientis |
recombinant His-tagged enzyme from Escherichia coli train BL21(DE3) by nickel affinity chromatography | Eubacterium oxidoreducens |
recombinant His-tagged enzyme from Escherichia coli train BL21(DE3) by nickel affinity chromatography | Clostridium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4,6-trihydroxyacetophenone + NADPH + H+ | - |
Desulfosporosinus orientis | 2-acetyl-3,5-dihydroxycyclohex-2-en-1-one + NADP+ | - |
? | |
2,4,6-trihydroxyacetophenone + NADPH + H+ | - |
Eubacterium oxidoreducens | 2-acetyl-3,5-dihydroxycyclohex-2-en-1-one + NADP+ | - |
? | |
2,4,6-trihydroxyacetophenone + NADPH + H+ | - |
Clostridium sp. | 2-acetyl-3,5-dihydroxycyclohex-2-en-1-one + NADP+ | - |
? | |
2,4,6-trihydroxyacetophenone + NADPH + H+ | - |
Eubacterium oxidoreducens AB3007 | 2-acetyl-3,5-dihydroxycyclohex-2-en-1-one + NADP+ | - |
? | |
2,4,6-trihydroxybenzaldehyde + NADPH + H+ | - |
Desulfosporosinus orientis | 2,4-dihydroxy-6-oxocyclohex-1-ene-1-carbaldehyde + NADP+ | - |
? | |
2,4,6-trihydroxybenzaldehyde + NADPH + H+ | - |
Eubacterium oxidoreducens | 2,4-dihydroxy-6-oxocyclohex-1-ene-1-carbaldehyde + NADP+ | - |
? | |
2,4,6-trihydroxybenzaldehyde + NADPH + H+ | - |
Clostridium sp. | 2,4-dihydroxy-6-oxocyclohex-1-ene-1-carbaldehyde + NADP+ | - |
? | |
2,4,6-trihydroxybenzaldehyde + NADPH + H+ | - |
Eubacterium oxidoreducens AB3007 | 2,4-dihydroxy-6-oxocyclohex-1-ene-1-carbaldehyde + NADP+ | - |
? | |
methyl 2,4,6-trihydroxybenzoate + NADPH + H+ | - |
Desulfosporosinus orientis | methyl 2,4-dihydroxy-6-oxocyclohex-1-ene-1-carboxylate + NADP+ | - |
? | |
methyl 2,4,6-trihydroxybenzoate + NADPH + H+ | - |
Eubacterium oxidoreducens | methyl 2,4-dihydroxy-6-oxocyclohex-1-ene-1-carboxylate + NADP+ | - |
? | |
methyl 2,4,6-trihydroxybenzoate + NADPH + H+ | - |
Clostridium sp. | methyl 2,4-dihydroxy-6-oxocyclohex-1-ene-1-carboxylate + NADP+ | - |
? | |
additional information | analysis of substrate speccificity, enzymatic dearomatization of monoaromatic phenol derivatives, narrow substrate range, overview. No activity with 26 different compounds, including bicyclic compounds such as flaviolin and 2-tetralone, and several aromatic and nonaromatic monocyclic compounds | Clostridium sp. | ? | - |
? | |
additional information | analysis of substrate specificity, enzymatic dearomatization of monoaromatic phenol derivatives, narrow substrate range, overview. No activity with 26 different compounds, including bicyclic compounds such as flaviolin and 2-tetralone, and several aromatic and nonaromatic monocyclic compounds | Desulfosporosinus orientis | ? | - |
? | |
additional information | analysis of substrate specificity, enzymatic dearomatization of monoaromatic phenol derivatives, narrow substrate range, overview. No activity with methyl 2,4,6-trihydroxybenzoate and with 26 different compounds, including bicyclic compounds such as flaviolin and 2-tetralone, and several aromatic and nonaromatic monocyclic compounds | Eubacterium oxidoreducens | ? | - |
? | |
additional information | analysis of substrate specificity, enzymatic dearomatization of monoaromatic phenol derivatives, narrow substrate range, overview. No activity with methyl 2,4,6-trihydroxybenzoate and with 26 different compounds, including bicyclic compounds such as flaviolin and 2-tetralone, and several aromatic and nonaromatic monocyclic compounds | Eubacterium oxidoreducens AB3007 | ? | - |
? | |
phloroglucinol + NADPH + H+ | - |
Desulfosporosinus orientis | dihydrophloroglucinol + NADP+ | - |
? | |
phloroglucinol + NADPH + H+ | - |
Eubacterium oxidoreducens | dihydrophloroglucinol + NADP+ | - |
? | |
phloroglucinol + NADPH + H+ | - |
Clostridium sp. | dihydrophloroglucinol + NADP+ | - |
? | |
phloroglucinol + NADPH + H+ | conversion rate of 30%, 1HNMR analysis of the product | Desulfosporosinus orientis | dihydrophloroglucinol + NADP+ | - |
? | |
phloroglucinol + NADPH + H+ | conversion rate of 36%, 1HNMR analysis of the product | Eubacterium oxidoreducens | dihydrophloroglucinol + NADP+ | - |
? | |
phloroglucinol + NADPH + H+ | conversion rate of 37%, 1HNMR analysis of the product | Clostridium sp. | dihydrophloroglucinol + NADP+ | - |
? | |
phloroglucinol + NADPH + H+ | - |
Eubacterium oxidoreducens AB3007 | dihydrophloroglucinol + NADP+ | - |
? | |
phloroglucinol + NADPH + H+ | conversion rate of 36%, 1HNMR analysis of the product | Eubacterium oxidoreducens AB3007 | dihydrophloroglucinol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 39000, about | Eubacterium oxidoreducens |
homotetramer | 4 * 30000, about | Clostridium sp. |
Synonyms | Comment | Organism |
---|---|---|
PGR | - |
Desulfosporosinus orientis |
PGR | - |
Eubacterium oxidoreducens |
PGR | - |
Clostridium sp. |
PGRcl | - |
Clostridium sp. |
PGRde | - |
Desulfosporosinus orientis |
PGReu | - |
Eubacterium oxidoreducens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | Rossmann fold for cofactor binding | Desulfosporosinus orientis | |
NADPH | Rossmann fold for cofactor binding | Eubacterium oxidoreducens | |
NADPH | the NADPH binding motif is G24-X-X-G27-X-X-G30, Rossmann fold for cofactor binding | Clostridium sp. |
General Information | Comment | Organism |
---|---|---|
evolution | phloroglucinol reductases, PGRs, are members of the family of NAD(P)H-dependent short-chain dehydrogenases/reductases (SDRs), PGR subfamily. Several amino acid positions are identified as being conserved within the PGR subfamily and might be involved in substrate differentiation | Desulfosporosinus orientis |
evolution | phloroglucinol reductases, PGRs, are members of the family of NAD(P)H-dependent short-chain dehydrogenases/reductases (SDRs), PGR subfamily. Several amino acid positions were identified as being conserved within the PGR subfamily and might be involved in substrate differentiation | Eubacterium oxidoreducens |
evolution | phloroglucinol reductases, PGRs, are members of the family of NAD(P)H-dependent short-chain dehydrogenases/reductases (SDRs), PGR subfamily. Several amino acid positions were identified as being conserved within the PGR subfamily and might be involved in substrate differentiation | Clostridium sp. |
metabolism | phloroglucinol is a central intermediate in the degradation of flavonoids and tannins, overview. It is further degraded to acetate and butyrate units | Desulfosporosinus orientis |
metabolism | phloroglucinol is a central intermediate in the degradation of flavonoids and tannins, overview. It is further degraded to acetate and butyrate units | Eubacterium oxidoreducens |
metabolism | phloroglucinol is a central intermediate in the degradation of flavonoids and tannins, overview. It is further degraded to acetate and butyrate units | Clostridium sp. |