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Literature summary for 1.3.1.70 extracted from

  • Kim, C.K.; Jeon, K.I.; Lim, D.M.; Johng, T.N.; Trzaskos, J.M.; Gaylor, J.L.; Paik, Y.K.
    Cholesterol biosynthesis from lanosterol: regulation and purification of rat hepatic sterol 14-reductase (1995), Biochim. Biophys. Acta, 1259, 39-48.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cholestyramine 11fold induction and activation by feeding 5% cholestyramine plus 0.1% lovastatin, CL-diet. 2fold induction and activation by feeding 5% cholestyramine alone Rattus norvegicus
liposome activity of purified enzyme totally dependent on the presence of liposome and NADPH Rattus norvegicus
lovastatin 11fold induction and activation by feeding 5% cholestyramine plus 0.1% lovastatin, CL-diet. 4fold induction and activation by feeding 0.1% lovastatin alone Rattus norvegicus
additional information the 2.5fold increase in enzymic activity due to the diurnal rhythm is completely abolished by cycloheximide Rattus norvegicus

General Stability

General Stability Organism
enzyme very labile Rattus norvegicus
very labile membrane-bound enzyme, totally dependent on the presence of liposome and NADPH Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
25-hydroxy-cholesterol IC50: 0.45 mM Rattus norvegicus
3beta-(2-(diethylamino)ethoxy)androst-5-en-17-one U18666A, IC50: 0.004 mM Rattus norvegicus
cholesterol enzyme suppressed by feeding 5% cholesterol, 70% decrease in enzyme activity Rattus norvegicus
cycloheximide the 2.5fold increase in enzymic activity due to the diurnal rhythm is completely abolished by cycloheximide Rattus norvegicus
miconazole IC50: 0.15 mM Rattus norvegicus
additional information kinetic pattern of inhibition; no inhibition by lovastatin, mevalonolactone, Squalestatin 1, (E)-N-ethyl-N-(6,6-dimethyl-2-hepten-4-ynyl)-3-((3,3'-bithiophen-5-yl)methoxy)benzenemethanamine, NB-598 Rattus norvegicus
trans-1,4-bis(2-chlorobenzylaminomethyl)cyclohexane dihydrochloride 0.01% AY-9944: suppression and inhibition; AY-9944, competitive inhibitor, strong inhibition, Ki: 0.00026; AY-9944, direct inhibitory effects of sterol 14-reductase; hepatocyte, IC50: 0.00025 mM, microsomes, IC50: 0.0003 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic pattern of inhibition Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Rattus norvegicus 16020
-
microsome microsomal-bound Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, two equally-sized subunits, SDS-PAGE Rattus norvegicus
70000
-
fast performance liquid chromatography Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus catalyzes anaerobically NADPH-dependent reduction of 14-double bond of 8,14-diene or 7,14-diene sterols that are sterol intermediates formed during C-32 demethylation in cholesterol biosynthesis from lanosterol in mammals ?
-
?
additional information Rattus norvegicus 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway ?
-
?
additional information Rattus norvegicus important essential enzyme of cholesterol biosynthesis from lanosterol ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
male sprague-dawley rats
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H+ regulation, regulatory mechanism, enzyme induction and suppression, dietary induction Rattus norvegicus
4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H+ reduction of 14-double bond of conjugated DELTA8,14-sterols and DELTA7,14-sterols Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0528
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,4-dimethyl-5alpha-cholesta-7,14-dien-3beta-ol + NADPH
-
Rattus norvegicus 4,4-dimethyl-5alpha-cholesta-7-en-3beta-ol + NADP+
-
?
additional information enzyme acts on a range of steroids with a 14(15)-double bond Rattus norvegicus ?
-
?
additional information catalyzes anaerobically NADPH-dependent reduction of 14-double bond of 8,14-diene or 7,14-diene sterols that are sterol intermediates formed during C-32 demethylation in cholesterol biosynthesis from lanosterol in mammals Rattus norvegicus ?
-
?
additional information 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway Rattus norvegicus ?
-
?
additional information important essential enzyme of cholesterol biosynthesis from lanosterol Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 38000, two equally-sized subunits, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at, anaerobically Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADPH NADPH-dependent Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0003
-
hepatocyte, IC50: 0.00025 mM, microsomes, IC50: 0.0003 mM Rattus norvegicus trans-1,4-bis(2-chlorobenzylaminomethyl)cyclohexane dihydrochloride
0.004
-
U18666A, IC50: 0.004 mM Rattus norvegicus 3beta-(2-(diethylamino)ethoxy)androst-5-en-17-one
0.15
-
IC50: 0.15 mM Rattus norvegicus miconazole
0.45
-
IC50: 0.45 mM Rattus norvegicus 25-hydroxy-cholesterol