BRENDA - Enzyme Database show
show all sequences of 1.3.1.70

Candida albicans sterol C-14 reductase, encoded by the ERG24 gene, as a potential antifungal target site

Jia, N.; Arthington-Skaggs, B.; Lee, W.; Pierson, C.A.; Lees, N.D.; Eckstein, J.; Barbuch, R.; Bard, M.; Antimicrob. Agents Chemother. 46, 947-957 (2002)

Data extracted from this reference:

Application
Application
Commentary
Organism
pharmacology
enzyme is a potential antifungal target site, development of antifungal compounds
Candida albicans
Cloned(Commentary)
Commentary
Organism
gene erg24, DNA and amino acid sequence determination, subcloning in Escherichia coli strain DH5alpha, complementation of the enzyme-deficient Saccharomyces cerevisiae erg24 mutant strain AP2
Candida albicans
Engineering
Amino acid exchange
Commentary
Organism
additional information
erg24 gene disruption mutant strains, i.e. strain NJ25, NJ50, NJ51, and NJ55, show growth, but altered phenotypes, e.g. increased sensitivity against an allylamine antifungal compound, overview
Candida albicans
Inhibitors
Inhibitors
Commentary
Organism
Structure
brefeldin
inhibition of growth of mutant strains NJ25, NJ50, NJ51, and NJ55, not of wild-type strain and mutant NJ25, overview
Candida albicans
Ca2+
slight inhibition of growth of the wild-type strain, increased inhibition of growth of erg24 mutant strains
Candida albicans
cerulenin
inhibition of growth of mutant strains NJ25, NJ50, NJ51, and NJ55, not of wild-type strain and mutant NJ25, overview
Candida albicans
clotrimazole
inhibition of growth of wild-type and mutant strains NJ25, NJ50, NJ51, and NJ55, overview
Candida albicans
cycloheximide
inhibition of growth of mutant strains NJ25, NJ50, NJ51, and NJ55, not of wild-type strain and mutant NJ25, overview
Candida albicans
fenpropimorph
inhibition of growth of wild-type and mutant strains NJ25, NJ50, NJ51, and NJ55, overview
Candida albicans
fluconazole
inhibition of growth of wild-type and mutant strain NJ25, only slightly of mutant strains NJ50, NJ51, and NJ55, overview
Candida albicans
fluphenazine
inhibition of growth of mutant strains NJ25, NJ50, NJ51, and NJ55, not of wild-type strain and mutant NJ25, overview
Candida albicans
itraconazole
inhibition of growth of wild-type and mutant strain NJ25, only slightly of mutant strains NJ50, NJ51, and NJ55, overview
Candida albicans
ketoconazole
inhibition of growth of wild-type and mutant strains NJ25, NJ50, NJ51, and NJ55, overview
Candida albicans
Nystatin
inhibition of growth of wild-type and mutant strain NJ25, not of mutants strains NJ50, NJ51, and NJ55, overview
Candida albicans
terbinafine
inhibition of growth of mutant strains NJ25, NJ50, NJ51, and NJ55, not of wild-type strain and mutant NJ25, overview
Candida albicans
tridemorph
inhibition of growth of wild-type and mutant strains NJ25, NJ50, NJ51, and NJ55, overview
Candida albicans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Candida albicans
enzyme plays a key role in sterol biosynthesis, sterol content in vivo
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida albicans
-
gene erg24
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
growth of wild-type and disruption mutant strains with various media and temperature conditions, overview
Candida albicans
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme plays a key role in sterol biosynthesis, sterol content in vivo
654204
Candida albicans
?
-
-
-
-
Application (protein specific)
Application
Commentary
Organism
pharmacology
enzyme is a potential antifungal target site, development of antifungal compounds
Candida albicans
Cloned(Commentary) (protein specific)
Commentary
Organism
gene erg24, DNA and amino acid sequence determination, subcloning in Escherichia coli strain DH5alpha, complementation of the enzyme-deficient Saccharomyces cerevisiae erg24 mutant strain AP2
Candida albicans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
erg24 gene disruption mutant strains, i.e. strain NJ25, NJ50, NJ51, and NJ55, show growth, but altered phenotypes, e.g. increased sensitivity against an allylamine antifungal compound, overview
Candida albicans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
brefeldin
inhibition of growth of mutant strains NJ25, NJ50, NJ51, and NJ55, not of wild-type strain and mutant NJ25, overview
Candida albicans
Ca2+
slight inhibition of growth of the wild-type strain, increased inhibition of growth of erg24 mutant strains
Candida albicans
cerulenin
inhibition of growth of mutant strains NJ25, NJ50, NJ51, and NJ55, not of wild-type strain and mutant NJ25, overview
Candida albicans
clotrimazole
inhibition of growth of wild-type and mutant strains NJ25, NJ50, NJ51, and NJ55, overview
Candida albicans
cycloheximide
inhibition of growth of mutant strains NJ25, NJ50, NJ51, and NJ55, not of wild-type strain and mutant NJ25, overview
Candida albicans
fenpropimorph
inhibition of growth of wild-type and mutant strains NJ25, NJ50, NJ51, and NJ55, overview
Candida albicans
fluconazole
inhibition of growth of wild-type and mutant strain NJ25, only slightly of mutant strains NJ50, NJ51, and NJ55, overview
Candida albicans
fluphenazine
inhibition of growth of mutant strains NJ25, NJ50, NJ51, and NJ55, not of wild-type strain and mutant NJ25, overview
Candida albicans
itraconazole
inhibition of growth of wild-type and mutant strain NJ25, only slightly of mutant strains NJ50, NJ51, and NJ55, overview
Candida albicans
ketoconazole
inhibition of growth of wild-type and mutant strains NJ25, NJ50, NJ51, and NJ55, overview
Candida albicans
Nystatin
inhibition of growth of wild-type and mutant strain NJ25, not of mutants strains NJ50, NJ51, and NJ55, overview
Candida albicans
terbinafine
inhibition of growth of mutant strains NJ25, NJ50, NJ51, and NJ55, not of wild-type strain and mutant NJ25, overview
Candida albicans
tridemorph
inhibition of growth of wild-type and mutant strains NJ25, NJ50, NJ51, and NJ55, overview
Candida albicans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Candida albicans
enzyme plays a key role in sterol biosynthesis, sterol content in vivo
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
growth of wild-type and disruption mutant strains with various media and temperature conditions, overview
Candida albicans
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme plays a key role in sterol biosynthesis, sterol content in vivo
654204
Candida albicans
?
-
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-
-
Other publictions for EC 1.3.1.70
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746161
Huang
Fackel interacts with gibbere ...
Arabidopsis thaliana
Planta
245
939-950
2017
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2
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744648
Bartoli
Impaired cell proliferation i ...
Mus musculus
Cell Cycle
15
2164-2173
2016
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1
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2
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745882
Li
Structure of an integral memb ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Nature
517
104-107
2015
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1
1
11
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3
3
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725903
Liu
A sterol C-14 reductase encode ...
Fusarium graminearum
Microbiology
157
1665-1675
2011
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3
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2
2
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711302
Schiavoni
Activation of TM7SF2 promoter ...
Homo sapiens
Biochim. Biophys. Acta
1801
587-592
2010
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1
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2
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713446
Wang
Gonadotropin-regulated express ...
Oryctolagus cuniculus
Prostaglandins Other Lipid Mediat.
92
25-32
2010
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1
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1
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725863
Hata
Inhibition of ergosterol synth ...
Candida albicans
Med. Mycol.
48
613-621
2010
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723885
Liang
2-Amino-nonyl-6-methoxyl-tetra ...
Candida albicans
Acta Pharmacol. Sin.
30
1709
2009
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688770
Shah Alam Bhuiyan
Synthetically lethal interacti ...
Saccharomyces cerevisiae
Lipids
42
69-76
2007
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672378
Bennati
Sterol dependent regulation of ...
Homo sapiens
Biochim. Biophys. Acta
1761
677-685
2006
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1
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2
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22
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22
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4
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655352
Yamashita
Gonadotropin-induced DELTA14-r ...
Sus scrofa
Endocrinology
146
186-194
2005
1
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654166
Waterham
Autosomal recessive HEM/Greenb ...
Homo sapiens
Am. J. Hum. Genet.
72
1013-1017
2003
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657046
He
Sterols regulate development a ...
Arabidopsis thaliana
Plant Physiol.
131
1258-1269
2003
1
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288492
Roberti
Cloning and expression of ster ...
Bos taurus, Homo sapiens
Eur. J. Biochem.
269
283-290
2002
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2
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6
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2
4
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7
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1
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12
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654204
Jia
Candida albicans sterol C-14 r ...
Candida albicans
Antimicrob. Agents Chemother.
46
947-957
2002
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1
1
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1
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13
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656304
Prakash
Genes encoding chimeras of Neu ...
Homo sapiens, Neurospora crassa
J. Biosci.
27
105-112
2002
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2
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3
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2
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8
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656798
Prakash
The sterol C-14 reductase enco ...
Neurospora crassa
Mol. Genet. Genomics
266
787-795
2002
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1
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38
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1
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38
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288491
Kim
Cholesterol biosynthesis from ...
Rattus norvegicus
Biochim. Biophys. Acta
1259
39-48
1995
4
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2
7
1
2
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1
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1
1
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4
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7
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288494
Lorenz
Cloning, sequencing, and disru ...
Saccharomyces cerevisiae
DNA Cell Biol.
11
685-692
1992
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288493
Taton
Microsomal DELTA8,14-sterol DE ...
Embryophyta, Zea mays
Eur. J. Biochem.
185
605-614
1989
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1
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28
5
6
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28
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5
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16
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3
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4
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1
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1
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2
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3
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2
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-
-
3
6
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-
-
2
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-
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1
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-
-
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4
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1
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1
1
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1
1
2
15
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1
-
-
-
1
2
-
2
-
-
-
2
1
-
2
-
-
3
-
12
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5
4
1
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4
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-
-
1
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1
1
2
15
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1
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1
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2
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1
5
2
2
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6
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4
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1
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11
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1
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1
1
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1
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2
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1
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1
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5
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2
2
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6
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1
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11
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1
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1
1
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