BRENDA - Enzyme Database
show all sequences of 1.3.1.70

Sterol dependent regulation of human TM7SF2 gene expression: role of the encoded 3beta-hydroxysterol DELTA14-reductase in human cholesterol biosynthesis

Bennati, A.M.; Castelli, M.; Della Fazia, M.A.; Beccari, T.; Caruso, D.; Servillo, G.; Roberti, R.; Biochim. Biophys. Acta 1761, 677-685 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression of LBR in COS-1 cells; TM7SF2 expression in COS-1 cells
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
TM7SF2
Homo sapiens
5783
-
nuclear membrane
inner nuclear membrane, LBR
Homo sapiens
31965
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Homo sapiens
C14SR is encoded by the TM7SF2 gene. TM7SF2 but not LBR, gene expression is regulated by cell sterol levels in HepG2 (human) and H-35 (rat) hepatoma cells. Primary role of C14SR in human cholesterol biosynthesis
?
-
-
-
additional information
Homo sapiens
role of LBR in the cholesterol biosynthesis pathway is unclear
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adrenal gland
LBR; TM7SF2 shows the highest mRNA expression
Homo sapiens
-
bone marrow
highest expression of LBR
Homo sapiens
-
brain
foetal and adult, LBR
Homo sapiens
-
colon
LBR
Homo sapiens
-
heart
foetal and adult; in foetal heart the expression is much lower then in the adult heart, TM7SF2
Homo sapiens
-
kidney
foetal and adult, LBR; TM7SF2
Homo sapiens
-
leukocyte
peripheral blood leukocyte, LBR
Homo sapiens
-
liver
foetal and adult, LBR; TM7SF2
Homo sapiens
-
lung
foetal and adult, LBR
Homo sapiens
-
lymph node
LBR; TM7SF2
Homo sapiens
-
ovary
LBR
Homo sapiens
-
pancreas
LBR; TM7SF2
Homo sapiens
-
placenta
LBR
Homo sapiens
-
prostate
LBR
Homo sapiens
-
prostate gland
TM7SF2
Homo sapiens
-
skeletal muscle
LBR
Homo sapiens
-
spleen
foetal and adult, LBR; LBR
Homo sapiens
-
stomach
LBR; TM7SF2
Homo sapiens
-
testis
LBR; TM7SF2
Homo sapiens
-
thymus
foetal and adult, LBR; in foetal thymus expression is much higher than in the adult thymus, TM7SF2
Homo sapiens
-
thyroid gland
LBR; TM7SF2
Homo sapiens
-
uterus
LBR
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5alpha-cholesta-8,14-dien-3beta-ol + NADP+
LBR
672378
Homo sapiens
5alpha-cholesta-8-en-3beta-ol + NADP+
-
-
-
?
5alpha-cholesta-8,14-dien-3beta-ol + NADP+
TM7SF2
672378
Homo sapiens
5alpha-cholesta-8-en-3beta-ol + NADP+
-
-
-
?
additional information
C14SR is encoded by the TM7SF2 gene. TM7SF2 but not LBR, gene expression is regulated by cell sterol levels in HepG2 (human) and H-35 (rat) hepatoma cells. Primary role of C14SR in human cholesterol biosynthesis
672378
Homo sapiens
?
-
-
-
-
additional information
role of LBR in the cholesterol biosynthesis pathway is unclear
672378
Homo sapiens
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
3beta-hydroxysterol DELTA14-reductase
besides the endoplasmic reticulum 3beta-hydroxysterol DELTA14-reductase (C14SR) encoded by TM7SF2 gene, the lamin B receptor (LBR) of the inner nuclear membrane possesses 3beta-hydroxysterol DELTA14-reductase activity
Homo sapiens
C14SR
-
Homo sapiens
lamin B receptor
-
Homo sapiens
LBR
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of LBR in COS-1 cells; TM7SF2 expression in COS-1 cells
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
TM7SF2
Homo sapiens
5783
-
nuclear membrane
inner nuclear membrane, LBR
Homo sapiens
31965
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Homo sapiens
C14SR is encoded by the TM7SF2 gene. TM7SF2 but not LBR, gene expression is regulated by cell sterol levels in HepG2 (human) and H-35 (rat) hepatoma cells. Primary role of C14SR in human cholesterol biosynthesis
?
-
-
-
additional information
Homo sapiens
role of LBR in the cholesterol biosynthesis pathway is unclear
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adrenal gland
LBR; TM7SF2 shows the highest mRNA expression
Homo sapiens
-
bone marrow
highest expression of LBR
Homo sapiens
-
brain
foetal and adult, LBR
Homo sapiens
-
colon
LBR
Homo sapiens
-
heart
foetal and adult; in foetal heart the expression is much lower then in the adult heart, TM7SF2
Homo sapiens
-
kidney
foetal and adult, LBR; TM7SF2
Homo sapiens
-
leukocyte
peripheral blood leukocyte, LBR
Homo sapiens
-
liver
foetal and adult, LBR; TM7SF2
Homo sapiens
-
lung
foetal and adult, LBR
Homo sapiens
-
lymph node
LBR; TM7SF2
Homo sapiens
-
ovary
LBR
Homo sapiens
-
pancreas
LBR; TM7SF2
Homo sapiens
-
placenta
LBR
Homo sapiens
-
prostate
LBR
Homo sapiens
-
prostate gland
TM7SF2
Homo sapiens
-
skeletal muscle
LBR
Homo sapiens
-
spleen
foetal and adult, LBR; LBR
Homo sapiens
-
stomach
LBR; TM7SF2
Homo sapiens
-
testis
LBR; TM7SF2
Homo sapiens
-
thymus
foetal and adult, LBR; in foetal thymus expression is much higher than in the adult thymus, TM7SF2
Homo sapiens
-
thyroid gland
LBR; TM7SF2
Homo sapiens
-
uterus
LBR
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5alpha-cholesta-8,14-dien-3beta-ol + NADP+
LBR
672378
Homo sapiens
5alpha-cholesta-8-en-3beta-ol + NADP+
-
-
-
?
5alpha-cholesta-8,14-dien-3beta-ol + NADP+
TM7SF2
672378
Homo sapiens
5alpha-cholesta-8-en-3beta-ol + NADP+
-
-
-
?
additional information
C14SR is encoded by the TM7SF2 gene. TM7SF2 but not LBR, gene expression is regulated by cell sterol levels in HepG2 (human) and H-35 (rat) hepatoma cells. Primary role of C14SR in human cholesterol biosynthesis
672378
Homo sapiens
?
-
-
-
-
additional information
role of LBR in the cholesterol biosynthesis pathway is unclear
672378
Homo sapiens
?
-
-
-
-
Other publictions for EC 1.3.1.70
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746161
Huang
Fackel interacts with gibbere ...
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245
939-950
2017
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3
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2
2
-
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-
744648
Bartoli
Impaired cell proliferation i ...
Mus musculus
Cell Cycle
15
2164-2173
2016
-
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1
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1
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-
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2
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1
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5
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1
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2
2
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-
745882
Li
Structure of an integral memb ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Nature
517
104-107
2015
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1
1
11
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2
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2
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6
-
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1
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8
1
4
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1
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1
1
1
11
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2
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2
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1
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8
1
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3
3
-
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-
725903
Liu
A sterol C-14 reductase encode ...
Fusarium graminearum
Microbiology
157
1665-1675
2011
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3
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2
2
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711302
Schiavoni
Activation of TM7SF2 promoter ...
Homo sapiens
Biochim. Biophys. Acta
1801
587-592
2010
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1
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1
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2
1
1
2
-
-
713446
Wang
Gonadotropin-regulated express ...
Oryctolagus cuniculus
Prostaglandins Other Lipid Mediat.
92
25-32
2010
-
-
1
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4
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1
1
1
1
-
-
725863
Hata
Inhibition of ergosterol synth ...
Candida albicans
Med. Mycol.
48
613-621
2010
-
-
1
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2
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5
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723885
Liang
2-Amino-nonyl-6-methoxyl-tetra ...
Candida albicans
Acta Pharmacol. Sin.
30
1709
2009
-
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1
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4
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1
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1
-
-
688770
Shah Alam Bhuiyan
Synthetically lethal interacti ...
Saccharomyces cerevisiae
Lipids
42
69-76
2007
-
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2
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672378
Bennati
Sterol dependent regulation of ...
Homo sapiens
Biochim. Biophys. Acta
1761
677-685
2006
-
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1
-
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2
-
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2
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3
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22
-
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4
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4
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1
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2
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2
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22
-
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4
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655352
Yamashita
Gonadotropin-induced DELTA14-r ...
Sus scrofa
Endocrinology
146
186-194
2005
1
-
-
-
-
-
1
-
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2
-
2
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4
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2
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1
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4
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2
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-
654166
Waterham
Autosomal recessive HEM/Greenb ...
Homo sapiens
Am. J. Hum. Genet.
72
1013-1017
2003
-
-
1
-
1
-
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2
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1
2
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4
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1
1
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1
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657046
He
Sterols regulate development a ...
Arabidopsis thaliana
Plant Physiol.
131
1258-1269
2003
1
-
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1
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1
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3
-
3
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1
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5
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1
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1
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5
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288492
Roberti
Cloning and expression of ster ...
Bos taurus, Homo sapiens
Eur. J. Biochem.
269
283-290
2002
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2
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6
-
2
4
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7
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1
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3
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12
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1
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1
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2
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2
2
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6
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2
4
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1
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3
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12
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1
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1
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-
654204
Jia
Candida albicans sterol C-14 r ...
Candida albicans
Antimicrob. Agents Chemother.
46
947-957
2002
-
1
1
-
1
-
13
-
-
-
-
1
-
5
-
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1
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1
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1
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1
1
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1
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13
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1
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1
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1
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656304
Prakash
Genes encoding chimeras of Neu ...
Homo sapiens, Neurospora crassa
J. Biosci.
27
105-112
2002
-
-
2
-
3
-
-
-
-
-
-
2
-
8
-
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-
1
-
1
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2
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3
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656798
Prakash
The sterol C-14 reductase enco ...
Neurospora crassa
Mol. Genet. Genomics
266
787-795
2002
-
-
1
-
38
-
1
-
-
-
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1
-
3
-
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1
-
1
-
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2
-
1
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1
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1
1
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38
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1
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2
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288491
Kim
Cholesterol biosynthesis from ...
Rattus norvegicus
Biochim. Biophys. Acta
1259
39-48
1995
4
-
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2
7
1
2
-
2
3
-
3
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1
1
-
3
1
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5
1
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1
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1
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4
4
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1
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2
4
7
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1
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2
3
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3
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12
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11
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