BRENDA - Enzyme Database show
show all sequences of 1.3.1.70

Impaired cell proliferation in regenerating liver of 3beta-hydroxysterol DELTA14-reductase (TM7SF2) knock-out mice

Bartoli, D.; Piobbico, D.; Bellet, M.M.; Bennati, A.M.; Roberti, R.; Della Fazia, M.A.; Servillo, G.; Cell Cycle 15, 2164-2173 (2016)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
generation of gene Tm7sf2 knockout mice
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Mus musculus
5783
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
Q71KT5
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Mus musculus
-
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Mus musculus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
generation of gene Tm7sf2 knockout mice
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Mus musculus
5783
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Mus musculus
-
General Information
General Information
Commentary
Organism
malfunction
Tm7sf2 deficiency during liver regeneration alters lipid metabolism and generates a stress condition, which, in turn, transiently unbalances hepatocytes cell cycle progression. Tm7sf2 knockout mice show no alteration in cholesterol content, but accumulation and delayed catabolism of hepatic triglycerides is observed, resulting in persistent steatosis at all times post hepatectomy. Delayed cell cycle progression to the G1/S phase is observed in Tm7sf2 knockout mice, resulting in reduced cell division at the time points examined associated to abnormal endoplasmic reticulum stress response, leading to alteration in p53 content and, consequently, induction of p21 expression in Tm7sf2 knockout mice. Tm7sf2 knockout mice exhibit a high-degree of transient hepatic steatosis during liver regeneration after partial hepatectomy. Hepatocytes from Tm7sf2 KO mice have a defective progression through the G1/S phase, associated to delayed and/or reduced expression of central regulators of the cell cycle such as cyclin D1, cyclin A, cyclin E1 and CDK4
Mus musculus
physiological function
the enzyme catalyzes the reduction of C14-unsaturated sterols during cholesterol biosynthesis from lanosterol. Role of C14-SR in vivo during cell proliferation by evaluating liver regeneration in Tm7sf2 knockout and wild-type mice, overview
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
Tm7sf2 deficiency during liver regeneration alters lipid metabolism and generates a stress condition, which, in turn, transiently unbalances hepatocytes cell cycle progression. Tm7sf2 knockout mice show no alteration in cholesterol content, but accumulation and delayed catabolism of hepatic triglycerides is observed, resulting in persistent steatosis at all times post hepatectomy. Delayed cell cycle progression to the G1/S phase is observed in Tm7sf2 knockout mice, resulting in reduced cell division at the time points examined associated to abnormal endoplasmic reticulum stress response, leading to alteration in p53 content and, consequently, induction of p21 expression in Tm7sf2 knockout mice. Tm7sf2 knockout mice exhibit a high-degree of transient hepatic steatosis during liver regeneration after partial hepatectomy. Hepatocytes from Tm7sf2 KO mice have a defective progression through the G1/S phase, associated to delayed and/or reduced expression of central regulators of the cell cycle such as cyclin D1, cyclin A, cyclin E1 and CDK4
Mus musculus
physiological function
the enzyme catalyzes the reduction of C14-unsaturated sterols during cholesterol biosynthesis from lanosterol. Role of C14-SR in vivo during cell proliferation by evaluating liver regeneration in Tm7sf2 knockout and wild-type mice, overview
Mus musculus
Other publictions for EC 1.3.1.70
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746161
Huang
Fackel interacts with gibbere ...
Arabidopsis thaliana
Planta
245
939-950
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
744648
Bartoli
Impaired cell proliferation i ...
Mus musculus
Cell Cycle
15
2164-2173
2016
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
745882
Li
Structure of an integral memb ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Nature
517
104-107
2015
-
-
1
1
11
-
-
-
2
-
-
2
-
6
-
-
1
-
-
-
-
-
8
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
11
-
-
-
-
-
2
-
-
2
-
-
-
1
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
725903
Liu
A sterol C-14 reductase encode ...
Fusarium graminearum
Microbiology
157
1665-1675
2011
-
-
-
-
-
-
3
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
711302
Schiavoni
Activation of TM7SF2 promoter ...
Homo sapiens
Biochim. Biophys. Acta
1801
587-592
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
713446
Wang
Gonadotropin-regulated express ...
Oryctolagus cuniculus
Prostaglandins Other Lipid Mediat.
92
25-32
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
725863
Hata
Inhibition of ergosterol synth ...
Candida albicans
Med. Mycol.
48
613-621
2010
-
-
1
-
-
-
2
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723885
Liang
2-Amino-nonyl-6-methoxyl-tetra ...
Candida albicans
Acta Pharmacol. Sin.
30
1709
2009
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
688770
Shah Alam Bhuiyan
Synthetically lethal interacti ...
Saccharomyces cerevisiae
Lipids
42
69-76
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672378
Bennati
Sterol dependent regulation of ...
Homo sapiens
Biochim. Biophys. Acta
1761
677-685
2006
-
-
1
-
-
-
-
-
2
-
-
2
-
3
-
-
-
-
-
22
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
22
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655352
Yamashita
Gonadotropin-induced DELTA14-r ...
Sus scrofa
Endocrinology
146
186-194
2005
1
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654166
Waterham
Autosomal recessive HEM/Greenb ...
Homo sapiens
Am. J. Hum. Genet.
72
1013-1017
2003
-
-
1
-
1
-
-
-
2
-
1
2
-
4
-
-
-
-
-
1
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
2
-
1
2
-
-
-
-
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657046
He
Sterols regulate development a ...
Arabidopsis thaliana
Plant Physiol.
131
1258-1269
2003
1
-
-
-
1
-
1
-
-
-
-
3
-
3
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288492
Roberti
Cloning and expression of ster ...
Bos taurus, Homo sapiens
Eur. J. Biochem.
269
283-290
2002
-
-
2
-
-
-
-
-
6
-
2
4
-
7
-
-
1
-
-
3
-
-
12
-
1
-
-
-
1
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
6
-
2
4
-
-
-
1
-
3
-
-
12
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
654204
Jia
Candida albicans sterol C-14 r ...
Candida albicans
Antimicrob. Agents Chemother.
46
947-957
2002
-
1
1
-
1
-
13
-
-
-
-
1
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
13
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656304
Prakash
Genes encoding chimeras of Neu ...
Homo sapiens, Neurospora crassa
J. Biosci.
27
105-112
2002
-
-
2
-
3
-
-
-
-
-
-
2
-
8
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656798
Prakash
The sterol C-14 reductase enco ...
Neurospora crassa
Mol. Genet. Genomics
266
787-795
2002
-
-
1
-
38
-
1
-
-
-
-
1
-
3
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
38
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288491
Kim
Cholesterol biosynthesis from ...
Rattus norvegicus
Biochim. Biophys. Acta
1259
39-48
1995
4
-
-
-
-
2
7
1
2
-
2
3
-
3
-
-
1
1
-
3
1
-
5
1
1
-
-
-
-
-
-
1
-
-
4
4
-
-
1
-
-
2
4
7
-
1
2
-
2
3
-
-
-
1
-
3
1
-
5
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
288494
Lorenz
Cloning, sequencing, and disru ...
Saccharomyces cerevisiae
DNA Cell Biol.
11
685-692
1992
-
-
1
-
-
-
2
-
-
-
1
2
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
1
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288493
Taton
Microsomal DELTA8,14-sterol DE ...
Embryophyta, Zea mays
Eur. J. Biochem.
185
605-614
1989
-
1
-
-
-
-
28
5
6
-
-
5
-
4
-
-
-
2
-
1
-
-
16
-
1
-
-
-
2
3
-
4
-
-
5
-
1
-
4
-
-
-
5
28
-
5
6
-
-
5
-
-
-
-
-
1
-
-
16
-
1
-
-
-
2
3
-
-
-
-
-
-
-
-
288495
Baloch
-
Inhibition of sterol DELTA8 -> ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae NCYC 739, Ustilago maydis
Phytochemistry
26
663-668
1987
-
-
-
-
-
-
6
-
-
-
-
2
-
3
-
-
1
1
-
-
-
-
4
-
1
-
-
-
1
-
-
2
-
-
3
-
-
-
2
-
-
-
3
6
-
-
-
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288490
Paik
Microsomal enzymes of choleste ...
Rattus norvegicus
J. Biol. Chem.
259
13413-13423
1984
2
1
-
-
-
3
12
5
4
1
-
4
-
2
-
-
1
1
-
1
1
2
15
-
1
-
-
-
1
2
-
2
-
-
-
2
1
-
2
-
-
3
-
12
-
5
4
1
-
4
-
-
-
1
-
1
1
2
15
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
288489
Bottema
DELTA14-Sterol reductase in Sa ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae 3701B
Biochim. Biophys. Acta
531
301-307
1978
2
-
-
-
-
1
5
2
2
-
-
6
-
4
-
-
1
-
-
-
-
-
11
-
1
-
-
-
1
1
-
1
-
-
-
2
-
-
1
-
-
1
-
5
-
2
2
-
-
6
-
-
-
1
-
-
-
-
11
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-