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Literature summary for 1.3.1.72 extracted from
- Inhibition of cholesterol biosynthesis by DELTA22-unsaturated phytosterols via competitive inhibition of sterol DELTA24-reductase in mammalian cells (2002), Biochem. J., 366, 109-119.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,22-cholestedien-3beta-ol | desmosterol isomer, competitive inhibition | Homo sapiens |  |
| 5,22-cholestedien-3beta-ol | desmosterol isomer, competitive inhibition | Rattus norvegicus | |
| brassicasterol | DELTA22-unsaturated phytosterol, competitive inhibition | Homo sapiens | |
| brassicasterol | DELTA22-unsaturated phytosterol, competitive inhibition | Rattus norvegicus | |
| ergosterol | DELTA22-unsaturated phytosterol, competitive inhibition | Homo sapiens | |
| ergosterol | DELTA22-unsaturated phytosterol, competitive inhibition | Rattus norvegicus | |
| additional information | inhibition mechanism of DELTA22-unsaturated phytosterols | Homo sapiens | |
| additional information | inhibition mechanism of DELTA22-unsaturated phytosterols | Rattus norvegicus | |
| stigmasterol | DELTA22-unsaturated phytosterol, competitive inhibition | Homo sapiens | |
| stigmasterol | DELTA22-unsaturated phytosterol, competitive inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.3 | - |
desmosterol | pH 7.6, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Homo sapiens | 16020 | - |
| membrane | bound | Rattus norvegicus | 16020 | - |
| microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5alpha-cholesta-7,24-dien-3beta-ol + NADPH | Homo sapiens | step in the biosynthesis of cholesterol from lanosterol | 5alpha-cholest-7-en-3beta-ol + NADP+ | - |
? | |
| 5alpha-cholesta-7,24-dien-3beta-ol + NADPH | Rattus norvegicus | step in the biosynthesis of cholesterol from lanosterol | 5alpha-cholest-7-en-3beta-ol + NADP+ | - |
? | |
| cholesta-5,7,24-trien-3beta-ol + NADPH | Homo sapiens | step in the biosynthesis of cholesterol from lanosterol | cholest-5,7-dien-3beta-ol + NADP+ | - |
? | |
| cholesta-5,7,24-trien-3beta-ol + NADPH | Rattus norvegicus | step in the biosynthesis of cholesterol from lanosterol | cholest-5,7-dien-3beta-ol + NADP+ | - |
? | |
| desmosterol + NADPH | Homo sapiens | step in the biosynthesis of cholesterol from lanosterol | cholesterol + NADP+ | - |
? | |
| desmosterol + NADPH | Rattus norvegicus | step in the biosynthesis of cholesterol from lanosterol | cholesterol + NADP+ | - |
? | |
| lanosterol + NADPH | Homo sapiens | step in the biosynthesis of cholesterol from lanosterol | 24-dihydrolanosterol + NADP+ | - |
? | |
| lanosterol + NADPH | Rattus norvegicus | step in the biosynthesis of cholesterol from lanosterol | 24-dihydrolanosterol + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Caco-2 cell | intestinal mucosa cells | Homo sapiens | - |
| HL-60 cell | promyelocytic cells | Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5alpha-cholesta-7,24-dien-3beta-ol + NADPH | best substrate | Homo sapiens | 5alpha-cholest-7-en-3beta-ol + NADP+ | i.e.lathosterol | ? | |
| 5alpha-cholesta-7,24-dien-3beta-ol + NADPH | best substrate | Rattus norvegicus | 5alpha-cholest-7-en-3beta-ol + NADP+ | i.e.lathosterol | ? | |
| 5alpha-cholesta-7,24-dien-3beta-ol + NADPH | step in the biosynthesis of cholesterol from lanosterol | Homo sapiens | 5alpha-cholest-7-en-3beta-ol + NADP+ | - |
? | |
| 5alpha-cholesta-7,24-dien-3beta-ol + NADPH | step in the biosynthesis of cholesterol from lanosterol | Rattus norvegicus | 5alpha-cholest-7-en-3beta-ol + NADP+ | - |
? | |
| cholesta-5,7,24-trien-3beta-ol + NADPH | - |
Homo sapiens | cholest-5,7-dien-3beta-ol + NADP+ | - |
? | |
| cholesta-5,7,24-trien-3beta-ol + NADPH | - |
Rattus norvegicus | cholest-5,7-dien-3beta-ol + NADP+ | - |
? | |
| cholesta-5,7,24-trien-3beta-ol + NADPH | step in the biosynthesis of cholesterol from lanosterol | Homo sapiens | cholest-5,7-dien-3beta-ol + NADP+ | - |
? | |
| cholesta-5,7,24-trien-3beta-ol + NADPH | step in the biosynthesis of cholesterol from lanosterol | Rattus norvegicus | cholest-5,7-dien-3beta-ol + NADP+ | - |
? | |
| desmosterol + NADPH | - |
Homo sapiens | cholesterol + NADP+ | - |
? | |
| desmosterol + NADPH | - |
Rattus norvegicus | cholesterol + NADP+ | - |
? | |
| desmosterol + NADPH | step in the biosynthesis of cholesterol from lanosterol | Homo sapiens | cholesterol + NADP+ | - |
? | |
| desmosterol + NADPH | step in the biosynthesis of cholesterol from lanosterol | Rattus norvegicus | cholesterol + NADP+ | - |
? | |
| lanosterol + NADPH | - |
Homo sapiens | 24-dihydrolanosterol + NADP+ | - |
? | |
| lanosterol + NADPH | - |
Rattus norvegicus | 24-dihydrolanosterol + NADP+ | - |
? | |
| lanosterol + NADPH | step in the biosynthesis of cholesterol from lanosterol | Homo sapiens | 24-dihydrolanosterol + NADP+ | - |
? | |
| lanosterol + NADPH | step in the biosynthesis of cholesterol from lanosterol | Rattus norvegicus | 24-dihydrolanosterol + NADP+ | - |
? | |
| zymosterol + NADPH | - |
Homo sapiens | 5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
| zymosterol + NADPH | - |
Rattus norvegicus | 5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| desmosterol reductase | - |
Homo sapiens |
| desmosterol reductase | - |
Rattus norvegicus |
| lanosterol reductase | - |
Homo sapiens |
| lanosterol reductase | - |
Rattus norvegicus |
| sterol 24DELTA-reductase | - |
Homo sapiens |
| sterol 24DELTA-reductase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Homo sapiens |
| 7.6 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Homo sapiens | |
| NADPH | - |
Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0033 | - |
5,22-cholestedien-3beta-ol | pH 7.6, 37°C | Rattus norvegicus | |
| 0.037 | - |
ergosterol | pH 7.6, 37°C | Rattus norvegicus | |
| 0.041 | - |
stigmasterol | pH 7.6, 37°C | Rattus norvegicus | |
| 0.043 | - |
brassicasterol | pH 7.6, 37°C | Rattus norvegicus | |
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