Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.72 extracted from

  • Saito, M.; Takano, T.; Nishimura, T.; Kohara, M.; Tsukiyama-Kohara, K.
    3beta-hydroxysterol DELTA24-reductase on the surface of hepatitis C virus-related hepatocellular carcinoma cells can be a target for molecular targeting therapy (2015), PLoS ONE, 10, e0124197 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine surface DHCR24 can be a valuable target for hepatitis C virus-related hepatocellular carcinoma therapy, and 2-152a MAb appears to be useful for this targeted therapy Homo sapiens
pharmacology 3beta-hydroxysterol DELTA24-reductase on the surface of hepatitis C virus-related hepatocellular carcinoma cells can be a target for molecular targeting therapy Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of a specific chimeric antibody 2-152a MAb Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface hepatocellular carcinoma cells, hepatoblastoma cell line, and cervical adenocarcinoma-derived cell line Homo sapiens 9986
-
endoplasmic reticulum membrane primarily, a putative transmembrane domain exists in the N-terminus of DHCR24 Homo sapiens 5789
-
additional information no DHCR24 expression on the surface of the normal human hepatocyte lines NKNT and TTNT Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q15392
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-
HEP-3B cell
-
Homo sapiens
-
Hep-G2 cell
-
Homo sapiens
-
hepatocellular carcinoma cell DHCR24 is specifically expressed on the surface of HCC cell lines Homo sapiens
-
Huh-7 cell
-
Homo sapiens
-

Synonyms

Synonyms Comment Organism
3beta-hydroxysterol DELTA24-reductase
-
Homo sapiens

Expression

Organism Comment Expression
Homo sapiens DHCR24 is overexpressed in hepatitis C virus-related hepatocellular carcinoma specifically induced by hepatitis C virus up

General Information

General Information Comment Organism
malfunction 2-152a MAb-mediated binding of a cytotoxic agent (a saponin-conjugated secondary antibody) to surface DHCR24 leads to significant cytotoxicity. HCV replication can be suppressed by inhibiting DHCR24 with an enzymatic inhibitor Homo sapiens
physiological function surface DHCR24 on hepatocellular carcinoma cells can function as a carrier for internalization, e.g. of hepatitis C virus, HCV. DHCR24-mediated cholesterol biosynthesis plays a crucial role in the HCV life cycle. Antibody 2-152a MAb-mediated binding of 2ndAb-Sap to surface DHCR24 on hepatocellular carcinoma cells predicted to lead to internalization of 2ndAb-Sap and subsequent cell death Homo sapiens