Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally YFP-tagged wild-type enzyme in Arabidopsis thaliana mutant lines deficient in the enzyme in the endoplasmic reticulum and plasma membrane | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a diminuto/dwarf1 (dim) T-DNA insertion mutant. The dim mutant plants, similarly to the dwarf5-2, show a dwarf phenotype due to the deficiency in brassinosteroids, caused by a block in the D24-sterol-D24-reductase activity. Compared to the wild-type, the dim plants show reduced levels of campesterol and sitosterol associated to an increase of 24-methylene cholesterol and isofucosterol, their respective metabolic precursors and substrates of DIM, complementation experiments with dwarf5-2, dim, and ste1-1 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Arabidopsis thaliana | 5783 | - |
plasma membrane | - |
Arabidopsis thaliana | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q39085 | - |
- |
Arabidopsis thaliana Col-0 | Q39085 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DELTA5,7-avenasterol + NADPH + H+ | - |
Arabidopsis thaliana | DELTA5,7-sitosterol + NADP+ | - |
? | |
DELTA5,7-avenasterol + NADPH + H+ | - |
Arabidopsis thaliana Col-0 | DELTA5,7-sitosterol + NADP+ | - |
? | |
DELTA7-avenasterol + NADPH + H+ | - |
Arabidopsis thaliana | DELTA7-sitosterol + NADP+ | - |
? | |
DELTA7-avenasterol + NADPH + H+ | - |
Arabidopsis thaliana Col-0 | DELTA7-sitosterol + NADP+ | - |
? | |
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH + H+ | - |
Arabidopsis thaliana | ergosterol + NADP+ | - |
? | |
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH + H+ | - |
Arabidopsis thaliana Col-0 | ergosterol + NADP+ | - |
? | |
isofucosterol + NADPH + H+ | - |
Arabidopsis thaliana | sitosterol + NADP+ | - |
? | |
isofucosterol + NADPH + H+ | - |
Arabidopsis thaliana Col-0 | sitosterol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA24-sterol-DELTA24-reductase | - |
Arabidopsis thaliana |
DIM | - |
Arabidopsis thaliana |
DIMINUTO/DWARF1 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | the enzyme dim mutant plants, similarly to the dwarf5-2, show a dwarf phenotype due to the deficiency in brassinosteroids, caused by a block in the D24-sterol-D24-reductase activity. Compared to the wild-type, the dim plants show reduced levels of campesterol and sitosterol associated to an increase of 24-methylene cholesterol and isofucosterol, their respective metabolic precursors and substrates of DIM. Mutant dim seedlings have short hypocotyls, petioles, and roots. Leaves of dim are round, curly, and dark green in color. In adult flowering plants, dim shows extremely short inflorescences with small flowers and severely reduced fertility. Phenotype, overview | Arabidopsis thaliana |
metabolism | the enzyme is involved in the sterol biosynthetic pathway in Arabidopsis thaliana, overview | Arabidopsis thaliana |