Literature summary for 1.3.1.76 extracted from

Videira, M.A.M.; Lobo, S.A.L.; Sousa, F.L.; Saraiva, L.M.
Identification of the sirohaem biosynthesis pathway in Staphylococcus aureus (2020), FEBS J., 287, 1537-1553 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21Star(DE3)pLysS cells	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.34	-	NAD+	at pH 8.0 and 25°C	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
precorrin-2 + NAD+	Staphylococcus aureus	-	sirohydrochlorin + NADH + H+	-	?
precorrin-2 + NAD+	Staphylococcus aureus Newman	-	sirohydrochlorin + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	A0A0H3KAM1	-	-
Staphylococcus aureus Newman	A0A0H3KAM1	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+ Sepharose column chromatography	Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.062	-	at pH 8.0 and 25°C	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
precorrin-2 + NAD+	-	Staphylococcus aureus	sirohydrochlorin + NADH + H+	-	?
precorrin-2 + NAD+	-	Staphylococcus aureus Newman	sirohydrochlorin + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 25000, SDS-PAGE	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
CysG	-	Staphylococcus aureus
CysG2	-	Staphylococcus aureus
P2D	-	Staphylococcus aureus
SirC	-	Staphylococcus aureus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	dependent on	Staphylococcus aureus

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Release 2023.1 (January 2023)