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Literature summary for 1.3.1.76 extracted from

  • Pennington, J.; Kemp, M.; McGarry, L.; Chen, Y.; Stroupe, M.
    Siroheme synthase orients substrates for dehydrogenase and chelatase activities in a common active site (2020), Nat. Commun., 11, 864 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.76

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli LMG194 cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
S128A mutant enzyme bound to precorrin-2, sirohydrochlorin, and a cobalt-sirohydrochlorin product, hanging drop vapor diffusion method, using 100 mM MES pH 5.0, 500 mM NaCl, 1% (v/v) 2-mercaptoethanol and 10-20% (w/v) PEG4000 Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
D104A inactive Salmonella enterica subsp. enterica serovar Typhimurium
P133H inactive Salmonella enterica subsp. enterica serovar Typhimurium
R260A inactive Salmonella enterica subsp. enterica serovar Typhimurium
S128A the variant is more active for dehydrogenation and chelation than wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
precorrin-2 + NAD+ Salmonella enterica subsp. enterica serovar Typhimurium
-
 sirohydrochlorin + NADH + H+
-
 ?
sirohydrochlorin + Fe2+ Salmonella enterica subsp. enterica serovar Typhimurium
-
 siroheme + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium P25924
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
precorrin-2 + NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium sirohydrochlorin + NADH + H+
-
 ?
sirohydrochlorin + Fe2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium siroheme + H+
-
 ?

Synonyms

Synonyms Comment Organism
CysG
-
 Salmonella enterica subsp. enterica serovar Typhimurium
siroheme synthase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Salmonella enterica subsp. enterica serovar Typhimurium