Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a fusion protein with maltose-binding protein | Arabidopsis thaliana |
expression in Escherichia coli as a fusion protein with maltose-binding protein | Medicago truncatula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+)-catechin | 0.5 mM, 50% inhibition | Arabidopsis thaliana | |
(+/-)-dihydroquercetin | 0.025 mM | Arabidopsis thaliana | |
additional information | no inhibition by Na+ up to 400 mM | Arabidopsis thaliana | |
additional information | no inhibition by (+)-catechin and (+/-)-dihydroquercetin | Medicago truncatula | |
Na+ | above 200 mM | Medicago truncatula | |
quercetin | strong inhibition | Arabidopsis thaliana | |
quercetin | strong inhibition | Medicago truncatula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0129 | - |
cyanidin | pH 6.0 | Medicago truncatula | |
0.0145 | - |
pelargonidin | pH 6.0 | Medicago truncatula | |
0.0178 | - |
delphinidin | pH 8.0 | Arabidopsis thaliana | |
0.0498 | - |
delphinidin | pH 6.0 | Medicago truncatula | |
0.0528 | - |
pelargonidin | pH 8.0 | Arabidopsis thaliana | |
0.0739 | - |
cyanidin | pH 8.0 | Arabidopsis thaliana | |
0.45 | - |
NADPH | pH 6.0 | Medicago truncatula | |
0.94 | - |
NADH | pH 6.0 | Medicago truncatula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
anthocyanidin + NAD(P)H | Arabidopsis thaliana | enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins | 2,3-cis-flavan-3-ol + NAD(P)+ | - |
? | |
anthocyanidin + NAD(P)H | Medicago truncatula | enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins | 2,3-cis-flavan-3-ol + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Medicago truncatula | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-epicatechin + NADH + H+ | - |
Medicago truncatula | cyanidin + NAD+ | - |
r | |
anthocyanidin + NAD(P)H | enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins | Arabidopsis thaliana | 2,3-cis-flavan-3-ol + NAD(P)+ | - |
? | |
anthocyanidin + NAD(P)H | enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins | Medicago truncatula | 2,3-cis-flavan-3-ol + NAD(P)+ | - |
? | |
cyanidin + NADPH | - |
Arabidopsis thaliana | (-)-epicatechin + NAD(P)+ | - |
? | |
cyanidin + NADPH | preference of anthocyanidin substrates in decreasing order: cyanidin, pelargonidin and delphinidin | Medicago truncatula | (-)-epicatechin + NAD(P)+ | - |
? | |
delphinidin + 2 NADPH + H+ | - |
Arabidopsis thaliana | (-)-epigallocatechin + 2 NADP+ | - |
? | |
delphinidin + 2 NADPH + H+ | preference of anthocyanidin substrates in decreasing order: cyanidin, pelargonidin and delphinidin | Medicago truncatula | (-)-epigallocatechin + 2 NADP+ | - |
? | |
pelargonidin + 2 NADPH + H+ | - |
Arabidopsis thaliana | (-)-epiafzelechin + 2 NADP+ | - |
? | |
pelargonidin + 2 NADPH + H+ | preference of anthocyanidin substrates in decreasing order: cyanidin, pelargonidin and delphinidin | Medicago truncatula | (-)-epiafzelechin + 2 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ANR | - |
Arabidopsis thaliana |
ANR | - |
Medicago truncatula |
AtANR | - |
Arabidopsis thaliana |
MtANR | - |
Medicago truncatula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Medicago truncatula |
55 | - |
- |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
delphinidin | pH 8.0 | Arabidopsis thaliana | |
0.18 | - |
cyanidin | pH 6.0 | Medicago truncatula | |
1.045 | - |
cyanidin | pH 8.0 | Arabidopsis thaliana | |
1.34 | - |
delphinidin | pH 6.0 | Medicago truncatula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
in 50 mM citrate/phosphate buffer | Medicago truncatula |
6 | - |
in 50 mM MES buffer | Medicago truncatula |
8 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.9 | pH 7.0: about 40% of maximal activity, pH 8.9: about 90% of maximal activity | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | slight preference for NADPH over NADH | Arabidopsis thaliana | |
NADH | slight preference for NADPH over NADH | Medicago truncatula | |
NADPH | slight preference for NADPH over NADH | Arabidopsis thaliana | |
NADPH | slight preference for NADPH over NADH | Medicago truncatula |