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Literature summary for 1.3.1.77 extracted from

  • Kim, Y.B.; Thwe, A.A.; Kim, Y.; Li, X.; Cho, J.W.; Park, P.B.; Valan Arasu, M.; Abdullah Al-Dhabi, N.; Kim, S.J.; Suzuki, T.; Hyun Jho, K.; Park, S.U.
    Transcripts of anthocyanidin reductase and leucoanthocyanidin reductase and measurement of catechin and epicatechin in tartary buckwheat (2014), ScientificWorldJournal, 2014, 726567 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.77

Cloned(Commentary)

Cloned (Comment) Organism
gene anr, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, cloned from two cultivars, Hokkai T8 and T10, quantitative real-time RT-PCR enzyme expression analysis Fagopyrum tataricum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Fagopyrum tataricum 9507
-
cytosol
-
 Fagopyrum tataricum 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
anthocyanidin + 2 NADPH + H+ Fagopyrum tataricum
-
 epicatechin + 2 NADP+ ? ?

Organism

Organism UniProt Comment Textmining
Fagopyrum tataricum V9PJG5 cvs. Hokkai T8 and Hokkai T10
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Fagopyrum tataricum
-
hairy root
-
 Fagopyrum tataricum
-
leaf
-
 Fagopyrum tataricum
-
additional information gene expression of FtLAR during sprout development under dark and light conditions in cvs. T8 and T10, determination of catechin and epicatechin contents in sprouts, the expression pattern of anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) do not match the accumulation pattern of proanthocyanidins in different organs of the two cultivars Hokkai T8 and Hokkai T10. FtANR expression levels are higher in T8 than T10 under both conditions. Specifically, transcript level in T8 under dark condition is higher than that under light condition, although fluctuation pattern is observed in light condition. FtANR expression is increased from 0 to 3 days-after-sowing for T8 cultivar under dark condition and remained relatively constant on 6, 9, and 12 days-after-sowing. The catechin content is correlated with color pigment. Epicatechin content is affected by light in T8 only, whereas T10 remains unaffected by light. Flowers of T8 and T10 contain the highest levels of epicatechin, whereas the lowest amount of epicatechin is in the seeds at stage 3 in T8 Fagopyrum tataricum
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root
-
 Fagopyrum tataricum
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seed
-
 Fagopyrum tataricum
-
seedling
-
 Fagopyrum tataricum
-
sprout grown under light or dark condition Fagopyrum tataricum
-
stem
-
 Fagopyrum tataricum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
anthocyanidin + 2 NADPH + H+
-
 Fagopyrum tataricum epicatechin + 2 NADP+ ? ?

Subunits

Subunits Comment Organism
? x * 36800, about, sequence calculation Fagopyrum tataricum

Synonyms

Synonyms Comment Organism
ANR
-
 Fagopyrum tataricum
anthocyanidin reductase
-
 Fagopyrum tataricum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Fagopyrum tataricum

pI Value

Organism Comment pI Value Maximum pI Value
Fagopyrum tataricum sequence calculation
-
 5.42

General Information

General Information Comment Organism
metabolism the enzyme is important in biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin, the proanthocyanidin pathway exists as ametabolic channel associated with cellular membranes Fagopyrum tataricum
physiological function anthocyanidin reductase (ANR), together with leucoanthocyanidin reductase (LAR, EC 1.17.1.3), plays an important role in the monomeric units biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin in several plants. ANR and LAR levels in tartary buckwheat might be regulated by different mechanisms for catechin and epicatechin biosynthesis under light and dark conditions. The catechin content is correlated with color pigment in roots Fagopyrum tataricum