Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.78 extracted from

  • Bonner, C.A.; Jensen, R.A.; Gander, J.E.; Keyhani, N.O.
    A core catalytic domain of the TyrA protein family: arogenate dehydrogenase from Synechocystis (2004), Biochem. J., 382, 279-291.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.78

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Synechocystis sp. PCC 6803

General Stability

General Stability Organism
full activity is retained in repeated freeze-thaw cycles, when concentrations of 0.01 mg of protein per ml or more are maintained Synechocystis sp. PCC 6803

Inhibitors

Inhibitors Comment Organism Structure
2',5'-ADP
-
 Synechocystis sp. PCC 6803
L-Tyr
-
 Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 NADP+ pH 8.6, 25°C Synechocystis sp. PCC 6803
0.331
-
 L-arogenate pH 8.6, 25°C Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arogenate + NADP+ Chlorella sorokiniana enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ Porphyridium purpureum enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ Anabaena sp. enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ Synechocystis sp. enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ Fischerella sp. enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ Prochlorothrix hollandica enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ Synechocystis sp. PCC 6803 enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ Synechococcus elongatus PCC 6301 enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ Synechocystis sp. PCC 6902 enzyme of the arogenate pathway L-tyrosine + NADPH + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
 ATCC 29151
-
Chlorella sorokiniana
-
-
-
Fischerella sp.
-
 ATCC 29539
-
Porphyridium purpureum
-
-
-
Prochlorothrix hollandica
-
-
-
Synechococcus elongatus PCC 6301
-
-
-
Synechocystis sp.
-
-
-
Synechocystis sp. PCC 6803 P73906
-
-
Synechocystis sp. PCC 6902
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Synechocystis sp. PCC 6803

Reaction

Reaction Comment Organism Reaction ID
L-arogenate + NADP+ = L-tyrosine + NADPH + CO2 + H+ mechanism is probably steady-state random order Synechocystis sp. PCC 6803
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0024
-
-
 Chlorella sorokiniana
0.0024
-
-
 Prochlorothrix hollandica
0.0078
-
-
 Anabaena sp.
0.0097
-
-
 Porphyridium purpureum
0.0109
-
-
 Fischerella sp.
0.0115
-
-
 Synechococcus elongatus PCC 6301
0.0145
-
-
 Synechocystis sp.
220
-
-
 Synechocystis sp. PCC 6803

Storage Stability

Storage Stability Organism
purified enzyme is less stable during storage at 4°C than when maintained frozen at -20°C or at -70°C Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arogenate + NADP+ enzyme of the arogenate pathway Chlorella sorokiniana L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ enzyme of the arogenate pathway Porphyridium purpureum L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ enzyme of the arogenate pathway Anabaena sp. L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ enzyme of the arogenate pathway Synechocystis sp. L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ enzyme of the arogenate pathway Fischerella sp. L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ enzyme of the arogenate pathway Prochlorothrix hollandica L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ enzyme of the arogenate pathway Synechocystis sp. PCC 6803 L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ enzyme of the arogenate pathway Synechococcus elongatus PCC 6301 L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Chlorella sorokiniana L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Porphyridium purpureum L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Anabaena sp. L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Synechocystis sp. L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Fischerella sp. L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Prochlorothrix hollandica L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Synechocystis sp. PCC 6803 L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Synechococcus elongatus PCC 6301 L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ enzyme of the arogenate pathway Synechocystis sp. PCC 6902 L-tyrosine + NADPH + CO2
-
 ?
L-arogenate + NADP+ specific for, no activity with prephenate and NAD+ Synechocystis sp. PCC 6902 L-tyrosine + NADPH + CO2
-
 ?
additional information no activity with prephenate and NAD+ Chlorella sorokiniana ?
-
 ?
additional information no activity with prephenate and NAD+ Porphyridium purpureum ?
-
 ?
additional information no activity with prephenate and NAD+ Anabaena sp. ?
-
 ?
additional information no activity with prephenate and NAD+ Synechocystis sp. ?
-
 ?
additional information no activity with prephenate and NAD+ Fischerella sp. ?
-
 ?
additional information no activity with prephenate and NAD+ Prochlorothrix hollandica ?
-
 ?
additional information no activity with prephenate and NAD+ Synechocystis sp. PCC 6803 ?
-
 ?
additional information no activity with prephenate and NAD+ Synechococcus elongatus PCC 6301 ?
-
 ?
additional information no activity with prephenate and NAD+ Synechocystis sp. PCC 6902 ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Synechocystis sp. PCC 6803

Synonyms

Synonyms Comment Organism
TyrAa
-
 Chlorella sorokiniana
TyrAa
-
 Porphyridium purpureum
TyrAa
-
 Anabaena sp.
TyrAa
-
 Synechocystis sp.
TyrAa
-
 Fischerella sp.
TyrAa
-
 Prochlorothrix hollandica
TyrAa
-
 Synechocystis sp. PCC 6803
TyrAa
-
 Synechococcus elongatus PCC 6301

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28 30
-
 Synechocystis sp. PCC 6803

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
28 42 28-30°C: optimum, 42°C: 50% of maximal activity Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3 8.8
-
 Synechocystis sp. PCC 6803

Cofactor

Cofactor Comment Organism Structure
NADP+ no activity with NAD+ Chlorella sorokiniana
NADP+ no activity with NAD+ Porphyridium purpureum
NADP+ no activity with NAD+ Anabaena sp.
NADP+ no activity with NAD+ Synechocystis sp.
NADP+ no activity with NAD+ Fischerella sp.
NADP+ no activity with NAD+ Prochlorothrix hollandica
NADP+ no activity with NAD+ Synechocystis sp. PCC 6803
NADP+ no activity with NAD+ Synechococcus elongatus PCC 6301

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.078
-
 L-Tyr pH 8.6, 25°C, versus NADP+ Synechocystis sp. PCC 6803
0.089
-
 L-Tyr pH 8.6, 25°C, versus L-arogenate Synechocystis sp. PCC 6803