Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.85 extracted from

  • Erb, T.J.; Berg, I.A.; Brecht, V.; Mueller, M.; Fuchs, G.; Alber, B.E.
    Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway (2007), Proc. Natl. Acad. Sci. USA, 104, 10631-10636.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.85

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cereibacter sphaeroides 2.4.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 CO2 in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1
0.2
-
 (2S)-ethylmalonyl-CoA in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1
0.4
-
 crotonyl-CoA in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1
0.7
-
 NADPH in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1

Metals/Ions

Metals/Ions Comment Organism Structure
additional information comprehensive metal analysis of crotonyl-CoA carboxylase/reductase by plasma emission spectroscopy indicates the absence of any metals Methylorubrum extorquens AM1
additional information comprehensive metal analysis of crotonyl-CoA carboxylase/reductase by plasma emission spectroscopy indicates the absence of any metals Cereibacter sphaeroides 2.4.1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47700
-
 2 * 47700, gel filtration Cereibacter sphaeroides 2.4.1
105000
-
 gel filtration Cereibacter sphaeroides 2.4.1

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides 2.4.1
-
-
-
Methylorubrum extorquens AM1
-
-
-
Streptomyces coelicolor
-
 strain A3
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and Cibacron Blue 3GA agarose column chromatography Cereibacter sphaeroides 2.4.1

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetate-grown cell
-
 Cereibacter sphaeroides 2.4.1
-
culture condition:butyrate-grown cell
-
 Streptomyces coelicolor
-
culture condition:methanol-grown cell
-
 Methylorubrum extorquens AM1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + CO2 + NADPH + H+ crotonyl-CoA is the best substrate Streptomyces coelicolor (2S)-ethylmalonyl-CoA + NADP+
-
 r
crotonyl-CoA + CO2 + NADPH + H+ crotonyl-CoA is the best substrate Methylorubrum extorquens AM1 (2S)-ethylmalonyl-CoA + NADP+
-
 r
crotonyl-CoA + CO2 + NADPH + H+ crotonyl-CoA is the best substrate Cereibacter sphaeroides 2.4.1 (2S)-ethylmalonyl-CoA + NADP+
-
 r
crotonyl-CoA + NADPH + H+ reduction at low rate Streptomyces coelicolor butyryl-CoA + NADP+
-
 ir
crotonyl-CoA + NADPH + H+ reduction at low rate Methylorubrum extorquens AM1 butyryl-CoA + NADP+
-
 ir
crotonyl-CoA + NADPH + H+ reduction at low rate Cereibacter sphaeroides 2.4.1 butyryl-CoA + NADP+
-
 ir

Subunits

Subunits Comment Organism
homodimer 2 * 47700, gel filtration Cereibacter sphaeroides 2.4.1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
104
-
 (2S)-ethylmalonyl-CoA in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Streptomyces coelicolor
NADPH
-
 Methylorubrum extorquens AM1
NADPH
-
 Cereibacter sphaeroides 2.4.1

General Information

General Information Comment Organism
metabolism crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway Streptomyces coelicolor
metabolism crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway Methylorubrum extorquens AM1
metabolism crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway Cereibacter sphaeroides 2.4.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
520
-
 (2S)-ethylmalonyl-CoA in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1