Literature summary for 1.3.1.9 extracted from

Lu, J.Z.; Muench, S.P.; Allary, M.; Campbell, S.; Roberts, C.W.; Mui, E.; McLeod, R.L.; Rice, D.W.; Prigge, S.T.
Type I and type II fatty acid biosynthesis in Eimeria tenella: enoyl reductase activity and structure (2007), Parasitology, 134, 1949-1962.

Search for more literature for 1.3.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-Star(DE3) cells	Eimeria tenella

Inhibitors

Inhibitors	Comment	Organism	Structure
triclosan	potent inhibitor	Eimeria tenella

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	crotonyl-CoA	in 100 mM sodium phosphate (pH 7.5)	Eimeria tenella
0.06	-	NADH	in 100 mM sodium phosphate (pH 7.5)	Eimeria tenella

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	SDS-PAGE	Eimeria tenella

Organism

Organism	UniProt	Comment	Textmining
Eimeria tenella	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap metal chelating column chromatography, HiTrap SP FastFlow column chromatography, and amylose column chromatography	Eimeria tenella

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
crotonyl-CoA + NADH + H+	-	Eimeria tenella	butyryl-CoA + NAD+	-	?
trans-2-enoyl-ACP + NADH + H+	-	Eimeria tenella	acyl-ACP + NAD+	-	?

Subunits

Subunits	Comment	Organism
tetramer	native PAGE	Eimeria tenella

Synonyms

Synonyms	Comment	Organism
ENR	-	Eimeria tenella
NADH-dependent enoyl reductase	-	Eimeria tenella

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Eimeria tenella

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00006	-	-	Eimeria tenella	triclosan

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)