Cloned (Comment) | Organism |
---|---|
gene murB, Verrucomicrobium spinosum possesses a fusion open reading frame annotated by the locus tag (VspiD_010100018130). Recombinant expression in Escherichia coli strain Rosetta (DE3) pLysS. The ORF, which is predicted to encode the enzymes UDP-N-acetylenolpyruvoylglucosamine reductase (MurB) and UDP-N-acetylmuramate:L-alanine ligase (MurC) is cloned. In vivo analyses using functional complementation shows that the fusion gene is able to functionally complement Escherichia coli murB (mutant ST5 strain) and murC temperature sensitive mutants. The purified recombinant fusion enzyme (MurB/CVs) is endowed with UDP-N-acetylmuramate:L-alanine ligase activity. All attempts to demonstrate an in vitro UDP-N-acetylenolpyruvoylglucosamine reductase (MurB) activity are unsuccessful. Phylogenetic analysis reveals that this fusion enzyme can only be identified in specific lineages within the Verrucomicrobia phylum. The fusion gene is able to functionally complement two Escherichia coli strains that harbor mutations in the murB and murC genes | Verrucomicrobium spinosum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Verrucomicrobium spinosum | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH + H+ | Verrucomicrobium spinosum | the enzyme MurB catalyzes the reduction of the enolpyruvyl moiety of UDP-GlcNAc-enolpyruvate to lactyl ether to produce UDP-N-acetylmuramic acid | UDP-N-acetyl-alpha-D-muramate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Verrucomicrobium spinosum | - |
- |
- |
Verrucomicrobium spinosum DSM 4136 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH + H+ | - |
Verrucomicrobium spinosum | UDP-N-acetyl-alpha-D-muramate + NADP+ | - |
? | |
UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH + H+ | the enzyme MurB catalyzes the reduction of the enolpyruvyl moiety of UDP-GlcNAc-enolpyruvate to lactyl ether to produce UDP-N-acetylmuramic acid | Verrucomicrobium spinosum | UDP-N-acetyl-alpha-D-muramate + NADP+ | - |
? | |
UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH + H+ | - |
Verrucomicrobium spinosum DSM 4136 | UDP-N-acetyl-alpha-D-muramate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 87300, recombinant MurB/CVs fusion enzyme, SDS-PAGE | Verrucomicrobium spinosum |
More | domain mapping of the MurB/CVs fusion enzyme of Verrucomicrobium spinosum and the individual MurB and MurC enzymes from Escherichia coli | Verrucomicrobium spinosum |
Synonyms | Comment | Organism |
---|---|---|
MurB | - |
Verrucomicrobium spinosum |
MurBAb | - |
Verrucomicrobium spinosum |
UDP-N-acetylenolpyruvoylglucosamine reductase | - |
Verrucomicrobium spinosum |
VspiD_010100018130 | locus name | Verrucomicrobium spinosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Verrucomicrobium spinosum |
General Information | Comment | Organism |
---|---|---|
evolution | Verrucomicrobium spinosum possesses a fusion open reading frame annotated by the locus tag (VspiD_010100018130). The ORF, which is predicted to encode the enzymes UDP-N-acetylenolpyruvoylglucosamine reductase (MurB) and UDP-N-acetylmuramate:L-alanine ligase (MurC) is cloned. In vivo analyses using functional complementation shows that the fusion gene is able to complement Escherichia coli murB and murC temperature sensitive mutants. The purified recombinant fusion enzyme (MurB/CVs) is endowed with UDP-N-acetylmuramate:L-alanine ligase activity. All attempts to demonstrate an in vitro UDP-N-acetylenolpyruvoylglucosamine reductase (MurB) activity are unsuccessful. Phylogenetic analysis reveals that this fusion enzyme can only be identified in specific lineages within the Verrucomicrobia phylum | Verrucomicrobium spinosum |
metabolism | MurB is involved in cytoplasmic steps of peptidoglycan biosynthesis the peptidoglycan biosynthesis pathway, overview | Verrucomicrobium spinosum |