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Literature summary for 1.3.1.98 extracted from

  • Videau, P.; Rivers, O.S.; Ushijima, B.; Oshiro, R.T.; Kim, M.J.; Philmus, B.; Cozy, L.M.
    Mutation of the murC and murB genes impairs heterocyst differentiation in Anabaena sp. strain PCC 7120 (2016), J. Bacteriol., 198, 1196-1206 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene murB or alr5066, construction of plasmid pPJAV308 and recombinant expression of the enzyme in Escherichia coli, functional complementation of a MurB enzyme-deficient Escherchia coli mutant Anabaena sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of a murB null mutant strain UHM350, introduction of PpetE-murB (pPJAV329) into the murB mutant in the presence of copper is sufficient for complete segregation of the murB insertion and created a viable strain UHM350, which displays a growth defect and spontaneously lyses if not passaged every 2 weeks once a sufficient density is reached during plate-based culture containing copper. The pattern of functional heterocysts and average filament lengths is altered and impaired compared to the wild-type Anabaena sp.

Organism

Organism UniProt Comment Textmining
Anabaena sp. Q8YM74 i.e. Nostoc sp.
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Anabaena sp. PCC 7120 Q8YM74 i.e. Nostoc sp.
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Synonyms

Synonyms Comment Organism
alr5066
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 Anabaena sp.
MurB
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 Anabaena sp.
UDP-N-acetylenolpyruvoylglucosamine reductase
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 Anabaena sp.

General Information

General Information Comment Organism
malfunction cells depleted of either murC or murB expression fail to differentiate heterocysts under normally inducing conditions and display decreased filament integrity Anabaena sp.
physiological function the multicellular bacterium Anabaena, which differentiates a periodic pattern of specialized heterocyst cells, requires peptidoglycan synthesis by the murine ligase genes murC (alr5065) and murB (alr5066) for maintenance of patterned gene expression, filament integrity, and overall development. Recombinant expression of gene murB in an MurB enzyme-deficient Escherchia coli mutant can functionally complement the mutant strain. Gene murB depletion does not affect the pattern of patS expression Anabaena sp.