Cloned (Comment) | Organism |
---|---|
expression in HEK293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H296G | inactive mutant enzyme, shows no reduction of polyprenol | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a polyprenol + NADPH + H+ | Homo sapiens | the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis | a dolichol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9H8P0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a dolichol + NADP+ | reduction of the alpha-isoprene subunit of polyprenol | Homo sapiens | a polyprenol + NADPH + H+ | - |
? | |
a polyprenol + NADPH + H+ | the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis | Homo sapiens | a dolichol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SRD5A3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | mutations in the SRD5A3 gene results in mental retardation, ophthalmologic and cerebellar defects of the patients | Homo sapiens |
metabolism | the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis | Homo sapiens |