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Literature summary for 1.3.1.B13 extracted from

  • Cantagrel, V.; Lefeber, D.J.; Ng, B.G.; Guan, Z.; Silhavy, J.L.; Bielas, S.L.; Lehle, L.; Hombauer, H.; Adamowicz, M.; Swiezewska, E.; De Brouwer, A.P.; Bluemel, P.; Sykut-Cegielska, J.; Houliston, S.; Swistun, D.; Ali, B.R.; Dobyns, W.B.; Babovic-Vuksanovic, D.; van Bokhoven, H.; Wevers, R.A.; Raetz, C.
    SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder (2010), Cell, 142, 203-217.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK293T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H296G inactive mutant enzyme, shows no reduction of polyprenol Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a polyprenol + NADPH + H+ Homo sapiens the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis a dolichol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H8P0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a dolichol + NADP+ reduction of the alpha-isoprene subunit of polyprenol Homo sapiens a polyprenol + NADPH + H+
-
?
a polyprenol + NADPH + H+ the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis Homo sapiens a dolichol + NADP+
-
?

Synonyms

Synonyms Comment Organism
SRD5A3
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens

General Information

General Information Comment Organism
malfunction mutations in the SRD5A3 gene results in mental retardation, ophthalmologic and cerebellar defects of the patients Homo sapiens
metabolism the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis Homo sapiens