Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acriflavine | diminished by addition of FAD or FMN | Ipomoea batatas | |
L-galactono-gamma-lactone | substrate inhibition at concentration above 4.2 mM | Ipomoea batatas | |
p-chloromercuribenzoate | reduced by addition of 2-mercapthoethanol | Ipomoea batatas |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-galactono-gamma-lactone | - |
Ipomoea batatas |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Ipomoea batatas | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration | Ipomoea batatas |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 2 ferricytochrome c | Ipomoea batatas | terminal step in L-ascorbic acid biosynthesis | L-ascorbate + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea batatas | - |
sweet potato | - |
Purification (Comment) | Organism |
---|---|
low recovery | Ipomoea batatas |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Ipomoea batatas | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37000 | - |
purified enzyme | Ipomoea batatas |
Storage Stability | Organism |
---|---|
-80°C, 0.3 mg per ml protein, 0.1 M potassium phosphate, pH 7.4, 10% glycerol,2 mM glutathione, 2 month | Ipomoea batatas |
-80°C, diluted protein, 0.1 M potassium phosphate, pH 7.4, 10% glycerol,2 mM glutathione, 30% loss of activity after 1 month | Ipomoea batatas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 2 ferricytochrome c | terminal step in L-ascorbic acid biosynthesis | Ipomoea batatas | L-ascorbate + 2 ferrocytochrome c + 2 H+ | - |
? | |
L-galactono-1,4-lactone + ferricytochrome c | specific for L-galactono-gamma-lactone | Ipomoea batatas | L-ascorbate + ferrocytochrome c | - |
? | |
L-gulono-gamma-lactone + ferricytochrome | very low activity | Ipomoea batatas | D-ascorbate + ferricytochrome c | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56000, SDS-PAGE | Ipomoea batatas |
Synonyms | Comment | Organism |
---|---|---|
GLDHase | - |
Ipomoea batatas |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Ipomoea batatas |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
- |
Ipomoea batatas |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | probably | Ipomoea batatas |