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Literature summary for 1.3.2.3 extracted from

  • Ostergaard, J.; Persiau, G.; Davey, M.W.; Bauw, G.; Van Montagu, M.
    Isolation of a cDNA coding for L-galactono-gamma-lactone dehydrogenase, an enzyme involved in the biosynthesis of ascorbic acid in plants. Purification, characterization, cDNA cloning, and expression in yeast (1997), J. Biol. Chem., 272, 30009-30016.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Brassica oleracea var. botrytis

Inhibitors

Inhibitors Comment Organism Structure
iodoacetic acid
-
Brassica oleracea var. botrytis
L-galactono-gamma-lactone substrate inhibition at concentration above 32.6 mM Brassica oleracea var. botrytis
additional information no inhibition with riboflavin and lycorine Brassica oleracea var. botrytis
N-ethylmaleimide
-
Brassica oleracea var. botrytis
p-hydroxymercuribenzoic acid
-
Brassica oleracea var. botrytis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
L-galactono-gamma-lactone
-
Brassica oleracea var. botrytis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Brassica oleracea var. botrytis 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+, Ca2+ and Sr2+ have no effect on activity Brassica oleracea var. botrytis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
gel filtration Brassica oleracea var. botrytis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactono-1,4-lactone + 2 ferricytochrome c Brassica oleracea var. botrytis terminal step in L-ascorbic acid biosynthesis L-ascorbate + 2 ferrocytochrome c + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Brassica oleracea var. botrytis O47881
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Brassica oleracea var. botrytis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
extract of yeast expressing the recombinant enzyme Brassica oleracea var. botrytis
50800
-
purified enzyme Brassica oleracea var. botrytis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactono-1,4-lactone + 2 ferricytochrome c terminal step in L-ascorbic acid biosynthesis Brassica oleracea var. botrytis L-ascorbate + 2 ferrocytochrome c + 2 H+
-
?
L-galactono-1,4-lactone + ferricytochrome c specific for cytochrome c as electron acceptor Brassica oleracea var. botrytis L-ascorbate + ferrocytochrome c
-
?
L-galactono-1,4-lactone + ferricytochrome c native and recombinant from yeast Brassica oleracea var. botrytis L-ascorbate + ferrocytochrome c
-
?
L-galactono-1,4-lactone + ferricytochrome c specific for L-galactono-gamma-lactone Brassica oleracea var. botrytis L-ascorbate + ferrocytochrome c
-
?

Subunits

Subunits Comment Organism
monomer 1 * 56000, SDS-PAGE Brassica oleracea var. botrytis

Synonyms

Synonyms Comment Organism
GLDase
-
Brassica oleracea var. botrytis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Brassica oleracea var. botrytis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Brassica oleracea var. botrytis

pH Range

pH Minimum pH Maximum Comment Organism
6 8.8
-
Brassica oleracea var. botrytis

Cofactor

Cofactor Comment Organism Structure
flavin not Brassica oleracea var. botrytis