Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | about 50% residual activity in the presence of 1 M NaCl, about 30% residual activity in the presence of 2 M NaCl, about 10% residual activity in the presence of 4 M NaCl, about 3.5% residual activity in the presence of 6 M NaCl | Streptomyces noursei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
cyclo(L-Leu-L-Phe) | in 100 mM Tris/HCl buffer, pH 8.0, at 30°C | Streptomyces noursei | |
0.067 | - |
cyclo(L-Phe-L-His) | in 100 mM Tris/HCl buffer, pH 8.0, at 30°C | Streptomyces noursei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21066 | - |
x * 21066, calculated from amino acid sequence | Streptomyces noursei |
23000 | - |
x * 23000, SDS-PAGE | Streptomyces noursei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclo(L-Leu-L-Phe) + O2 | Streptomyces noursei | the catalytic reaction of the natural substrate cyclo(L-leucyl-L-phenylalanyl) occurs in a two-step sequential reaction leading first to cyclo(alpha,beta-dehydro-Phe-L-Leu) and finally to albonoursin | albonoursin + H2O2 | - |
? | |
cyclo(L-Leu-L-Phe) + O2 | Streptomyces noursei | - |
cyclo[(Z)-alpha,beta-didehydrophenylalanyl-L-leucyl] + H2O2 | - |
? | |
cyclo(L-Leu-L-Phe) + O2 | Streptomyces noursei ATCC 11455 | the catalytic reaction of the natural substrate cyclo(L-leucyl-L-phenylalanyl) occurs in a two-step sequential reaction leading first to cyclo(alpha,beta-dehydro-Phe-L-Leu) and finally to albonoursin | albonoursin + H2O2 | - |
? | |
cyclo(L-Leu-L-Phe) + O2 | Streptomyces noursei ATCC 11455 | - |
cyclo[(Z)-alpha,beta-didehydrophenylalanyl-L-leucyl] + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces noursei | Q8GED9 | - |
- |
Streptomyces noursei ATCC 11455 | Q8GED9 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, EMD propyl column chromatography, and Superose 6 gel filtration | Streptomyces noursei |
Storage Stability | Organism |
---|---|
-80°C, purified enzyme in the absence of any additive, several months, no loss of activity | Streptomyces noursei |
22°C, purified enzyme in the absence of any additive, 24 h, no loss of activity | Streptomyces noursei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclo(L-Glu-Gly) + O2 | 3% mono-dehydro-products and no bis-dehydro products | Streptomyces noursei | (4Z)-4-(3,6-dioxopiperazin-2-yl)butanoate + H2O2 | - |
? | |
cyclo(L-Glu-Gly) + O2 | 3% mono-dehydro-products and no bis-dehydro products | Streptomyces noursei ATCC 11455 | (4Z)-4-(3,6-dioxopiperazin-2-yl)butanoate + H2O2 | - |
? | |
cyclo(L-Leu-Gly) + O2 | 9% mono-dehydro-products and no bis-dehydro products | Streptomyces noursei | (3Z)-3-(2-methylpropylidene)piperazine-2,5-dione + H2O2 | - |
? | |
cyclo(L-Leu-Gly) + O2 | 9% mono-dehydro-products and no bis-dehydro products | Streptomyces noursei ATCC 11455 | (3Z)-3-(2-methylpropylidene)piperazine-2,5-dione + H2O2 | - |
? | |
cyclo(L-Leu-L-Ala) + O2 | 13% mono-dehydro-products and 4% bis-dehydro products | Streptomyces noursei | ? | - |
? | |
cyclo(L-Leu-L-Phe) + O2 | the catalytic reaction of the natural substrate cyclo(L-leucyl-L-phenylalanyl) occurs in a two-step sequential reaction leading first to cyclo(alpha,beta-dehydro-Phe-L-Leu) and finally to albonoursin | Streptomyces noursei | albonoursin + H2O2 | - |
? | |
cyclo(L-Leu-L-Phe) + O2 | the catalytic reaction of the natural substrate cyclo(L-leucyl-L-phenylalanyl) occurs in a two-step sequential reaction leading first to cyclo(alpha,beta-dehydro-Phe-L-Leu) and finally to albonoursin | Streptomyces noursei ATCC 11455 | albonoursin + H2O2 | - |
? | |
cyclo(L-Leu-L-Phe) + O2 | - |
Streptomyces noursei | cyclo[(Z)-alpha,beta-didehydrophenylalanyl-L-leucyl] + H2O2 | - |
? | |
cyclo(L-Leu-L-Phe) + O2 | - |
Streptomyces noursei ATCC 11455 | cyclo[(Z)-alpha,beta-didehydrophenylalanyl-L-leucyl] + H2O2 | - |
? | |
cyclo(L-Phe-Gly) + O2 | 36% mono-dehydro-products and no bis-dehydro products | Streptomyces noursei | (3Z)-3-benzylidenepiperazine-2,5-dione + H2O2 | - |
? | |
cyclo(L-Phe-L-His) + O2 | 21% mono-dehydro-products and less than 1% bis-dehydro products | Streptomyces noursei | ? | - |
? | |
cyclo(L-Ser-Gly) + O2 | 11% mono-dehydro-products and no bis-dehydro products | Streptomyces noursei | (3Z)-3-(2-hydroxyethylidene)piperazine-2,5-dione + H2O2 | - |
? | |
cyclo(L-Trp-L-Trp) + O2 | 74% mono-dehydro-products and 14% bis-dehydro products | Streptomyces noursei | ? | - |
? | |
cyclo[(Z)-alpha,beta-didehydrophenylalanyl-L-leucyl] + O2 | - |
Streptomyces noursei | albonoursin + H2O2 | - |
? | |
additional information | no activity towards N-acetyl-L-Phe-L-Leu and N-actely-L-Leu-L-Phe | Streptomyces noursei | ? | - |
? | |
additional information | no activity towards N-acetyl-L-Phe-L-Leu and N-actely-L-Leu-L-Phe | Streptomyces noursei ATCC 11455 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Streptomyces noursei |
? | x * 21066, calculated from amino acid sequence | Streptomyces noursei |
Synonyms | Comment | Organism |
---|---|---|
cyclic dipeptide oxidase | - |
Streptomyces noursei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
activity increases up to the maximum at 60°C followed by a substantial drop above this temperature | Streptomyces noursei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.453 | - |
cyclo(L-Phe-L-His) | in 100 mM Tris/HCl buffer, pH 8.0, at 30°C | Streptomyces noursei | |
0.69 | - |
cyclo(L-Leu-L-Phe) | in 100 mM Tris/HCl buffer, pH 8.0, at 30°C | Streptomyces noursei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Streptomyces noursei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | - |
Streptomyces noursei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.7 | the enzyme activity is quite stable at neutral and basic pH values, whereas it decreases at acidic pH values. Residual activity is 75% after 6.5 h at pH 6.0 | Streptomyces noursei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces noursei | isoelectric focusing | - |
3.8 |