Analysis of the biosynthesis of antibacterial cyclic dipeptides in Nocardiopsis alba
Li, Y.; Lai, Y.M.; Lu, Y.; Yang, Y.L.; Chen, S.; Arch. Microbiol. 196, 765-774 (2014) 
Data extracted from this reference:
Cloned(Commentary)
Cloned (Commentary)
Organism
the enzyme is encoded in the albonoursin biosynthetic gene cluster, albABC, genes albAB encode units for a cyclic dipeptide oxidase complex, responsible for generating two dehydrogenated double bonds
Nocardiopsis alba
Protein Variants
Protein Variants
Commentary
Organism
additional information
generation of an albABC gene deletion mutant YL001 of Nocardiopsis alba, genetic organization, overview. Strain YL001 show no significant difference from the wild-type in colonial morphology, and it has a slightly vibrant growth on the MS agar, production of the cyclic dipeptides is abolished in the DELTAalbABC mutant
Nocardiopsis alba
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
Nocardiopsis alba
-
? + 2 H2O2
-
-
?
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
Nocardiopsis alba ATCC BAA-2165
-
? + 2 H2O2
-
-
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
Nocardiopsis alba
-
albonoursin + 2 H2O2
-
-
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
Nocardiopsis alba ATCC BAA-2165
-
albonoursin + 2 H2O2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Nocardiopsis alba
-
isolated from the guts of healthy honeybees in a year-round survey
-
Nocardiopsis alba ATCC BAA-2165
-
isolated from the guts of healthy honeybees in a year-round survey
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
-
744181
Nocardiopsis alba
? + 2 H2O2
-
-
-
?
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
-
744181
Nocardiopsis alba ATCC BAA-2165
? + 2 H2O2
-
-
-
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
-
744181
Nocardiopsis alba
albonoursin + 2 H2O2
-
-
-
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
-
744181
Nocardiopsis alba ATCC BAA-2165
albonoursin + 2 H2O2
-
-
-
?
additional information
albonoursin is then methoxylated to cyclo(L-methoxytyrosine-L-leucine) by O-methyltranferases, encoded by genes B005_4460 and B005_4464, termed albM1 and albM2
744181
Nocardiopsis alba
?
-
-
-
?
additional information
albonoursin is then methoxylated to cyclo(L-methoxytyrosine-L-leucine) by O-methyltranferases, encoded by genes B005_4460 and B005_4464, termed albM1 and albM2
744181
Nocardiopsis alba ATCC BAA-2165
?
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
the enzyme is encoded in the albonoursin biosynthetic gene cluster, albABC, genes albAB encode units for a cyclic dipeptide oxidase complex, responsible for generating two dehydrogenated double bonds
Nocardiopsis alba
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
generation of an albABC gene deletion mutant YL001 of Nocardiopsis alba, genetic organization, overview. Strain YL001 show no significant difference from the wild-type in colonial morphology, and it has a slightly vibrant growth on the MS agar, production of the cyclic dipeptides is abolished in the DELTAalbABC mutant
Nocardiopsis alba
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
Nocardiopsis alba
-
? + 2 H2O2
-
-
?
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
Nocardiopsis alba ATCC BAA-2165
-
? + 2 H2O2
-
-
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
Nocardiopsis alba
-
albonoursin + 2 H2O2
-
-
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
Nocardiopsis alba ATCC BAA-2165
-
albonoursin + 2 H2O2
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
-
744181
Nocardiopsis alba
? + 2 H2O2
-
-
-
?
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
-
744181
Nocardiopsis alba ATCC BAA-2165
? + 2 H2O2
-
-
-
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
-
744181
Nocardiopsis alba
albonoursin + 2 H2O2
-
-
-
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
-
744181
Nocardiopsis alba ATCC BAA-2165
albonoursin + 2 H2O2
-
-
-
?
additional information
albonoursin is then methoxylated to cyclo(L-methoxytyrosine-L-leucine) by O-methyltranferases, encoded by genes B005_4460 and B005_4464, termed albM1 and albM2
744181
Nocardiopsis alba
?
-
-
-
?
additional information
albonoursin is then methoxylated to cyclo(L-methoxytyrosine-L-leucine) by O-methyltranferases, encoded by genes B005_4460 and B005_4464, termed albM1 and albM2
744181
Nocardiopsis alba ATCC BAA-2165
?
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
the albABC gene operon deletion mutant of Nocariopsis alba cannot produce cyclic dipeptides, cyclo(DELTAPhe-DELTALeu) i.e. albonoursin, and cyclo(DELTAmTyr-DELTALeu)
Nocardiopsis alba
metabolism
the albonoursin biosynthetic gene cluster is involved in the regulated biosynthesis of antibacterial cyclic dipeptides in Nocardiopsis alba
Nocardiopsis alba
physiological function
the enzyme is required for biosynthesis of antibacterial cyclic dipeptides, cyclo(DELTAPhe-DELTALeu) i.e. albonoursin, and cyclo(DELTAmTyr-DELTALeu)
Nocardiopsis alba
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the albABC gene operon deletion mutant of Nocariopsis alba cannot produce cyclic dipeptides, cyclo(DELTAPhe-DELTALeu) i.e. albonoursin, and cyclo(DELTAmTyr-DELTALeu)
Nocardiopsis alba
metabolism
the albonoursin biosynthetic gene cluster is involved in the regulated biosynthesis of antibacterial cyclic dipeptides in Nocardiopsis alba
Nocardiopsis alba
physiological function
the enzyme is required for biosynthesis of antibacterial cyclic dipeptides, cyclo(DELTAPhe-DELTALeu) i.e. albonoursin, and cyclo(DELTAmTyr-DELTALeu)
Nocardiopsis alba
Other publictions for EC 1.3.3.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
744181
Li
Analysis of the biosynthesis ...
Nocardiopsis alba, Nocardiopsis alba ATCC BAA-2165
Arch. Microbiol.
196
765-774
2014
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6
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3
3
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722021
Lautru
The albonoursin gene cluster o ...
Streptomyces noursei
Chem. Biol.
9
1355-1364
2002
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1
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1
1
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3
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1
1
1
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1
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1
1
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1
1
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722172
Gondry
Cyclic dipeptide oxidase from ...
Streptomyces noursei, Streptomyces noursei ATCC 11455
Eur. J. Biochem.
268
1712-1721
2001
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2
4
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5
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1
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2
16
2
1
1
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2
1
1
1
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1
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1
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2
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2
4
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1
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2
16
2
1
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2
1
1
1
1
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