Data extracted from this reference:
Cloned(Commentary)
the enzyme is encoded in the albonoursin biosynthetic gene cluster, albABC, genes albAB encode units for a cyclic dipeptide oxidase complex, responsible for generating two dehydrogenated double bonds
Nocardiopsis alba
Protein Variants
additional information
generation of an albABC gene deletion mutant YL001 of Nocardiopsis alba, genetic organization, overview. Strain YL001 show no significant difference from the wild-type in colonial morphology, and it has a slightly vibrant growth on the MS agar, production of the cyclic dipeptides is abolished in the DELTAalbABC mutant
Nocardiopsis alba
Natural Substrates/ Products (Substrates)
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
Nocardiopsis alba
? + 2 H2O2
?
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
Nocardiopsis alba ATCC BAA-2165
? + 2 H2O2
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
Nocardiopsis alba
albonoursin + 2 H2O2
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
Nocardiopsis alba ATCC BAA-2165
albonoursin + 2 H2O2
?
Organism
Nocardiopsis alba
isolated from the guts of healthy honeybees in a year-round survey
Nocardiopsis alba ATCC BAA-2165
isolated from the guts of healthy honeybees in a year-round survey
Substrates and Products (Substrate)
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
744181
Nocardiopsis alba
? + 2 H2O2
?
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
744181
Nocardiopsis alba ATCC BAA-2165
? + 2 H2O2
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
744181
Nocardiopsis alba
albonoursin + 2 H2O2
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
744181
Nocardiopsis alba ATCC BAA-2165
albonoursin + 2 H2O2
?
additional information
albonoursin is then methoxylated to cyclo(L-methoxytyrosine-L-leucine) by O-methyltranferases, encoded by genes B005_4460 and B005_4464, termed albM1 and albM2
744181
Nocardiopsis alba
?
?
additional information
albonoursin is then methoxylated to cyclo(L-methoxytyrosine-L-leucine) by O-methyltranferases, encoded by genes B005_4460 and B005_4464, termed albM1 and albM2
744181
Nocardiopsis alba ATCC BAA-2165
?
?
Cloned(Commentary) (protein specific)
the enzyme is encoded in the albonoursin biosynthetic gene cluster, albABC, genes albAB encode units for a cyclic dipeptide oxidase complex, responsible for generating two dehydrogenated double bonds
Nocardiopsis alba
Engineering (protein specific)
additional information
generation of an albABC gene deletion mutant YL001 of Nocardiopsis alba, genetic organization, overview. Strain YL001 show no significant difference from the wild-type in colonial morphology, and it has a slightly vibrant growth on the MS agar, production of the cyclic dipeptides is abolished in the DELTAalbABC mutant
Nocardiopsis alba
Natural Substrates/ Products (Substrates) (protein specific)
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
Nocardiopsis alba
? + 2 H2O2
?
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
Nocardiopsis alba ATCC BAA-2165
? + 2 H2O2
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
Nocardiopsis alba
albonoursin + 2 H2O2
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
Nocardiopsis alba ATCC BAA-2165
albonoursin + 2 H2O2
?
Substrates and Products (Substrate) (protein specific)
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
744181
Nocardiopsis alba
? + 2 H2O2
?
cyclo(L-4-O-methyltyrosine-L-leucine) + 2 O2
744181
Nocardiopsis alba ATCC BAA-2165
? + 2 H2O2
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
744181
Nocardiopsis alba
albonoursin + 2 H2O2
?
cyclo(L-leucyl-L-phenylalanyl) + 2 O2
744181
Nocardiopsis alba ATCC BAA-2165
albonoursin + 2 H2O2
?
additional information
albonoursin is then methoxylated to cyclo(L-methoxytyrosine-L-leucine) by O-methyltranferases, encoded by genes B005_4460 and B005_4464, termed albM1 and albM2
744181
Nocardiopsis alba
?
?
additional information
albonoursin is then methoxylated to cyclo(L-methoxytyrosine-L-leucine) by O-methyltranferases, encoded by genes B005_4460 and B005_4464, termed albM1 and albM2
744181
Nocardiopsis alba ATCC BAA-2165
?
?
General Information
malfunction
the albABC gene operon deletion mutant of Nocariopsis alba cannot produce cyclic dipeptides, cyclo(DELTAPhe-DELTALeu) i.e. albonoursin, and cyclo(DELTAmTyr-DELTALeu)
Nocardiopsis alba
metabolism
the albonoursin biosynthetic gene cluster is involved in the regulated biosynthesis of antibacterial cyclic dipeptides in Nocardiopsis alba
Nocardiopsis alba
physiological function
the enzyme is required for biosynthesis of antibacterial cyclic dipeptides, cyclo(DELTAPhe-DELTALeu) i.e. albonoursin, and cyclo(DELTAmTyr-DELTALeu)
Nocardiopsis alba
General Information (protein specific)
malfunction
the albABC gene operon deletion mutant of Nocariopsis alba cannot produce cyclic dipeptides, cyclo(DELTAPhe-DELTALeu) i.e. albonoursin, and cyclo(DELTAmTyr-DELTALeu)
Nocardiopsis alba
metabolism
the albonoursin biosynthetic gene cluster is involved in the regulated biosynthesis of antibacterial cyclic dipeptides in Nocardiopsis alba
Nocardiopsis alba
physiological function
the enzyme is required for biosynthesis of antibacterial cyclic dipeptides, cyclo(DELTAPhe-DELTALeu) i.e. albonoursin, and cyclo(DELTAmTyr-DELTALeu)
Nocardiopsis alba
Other publictions for EC 1.3.3.13
744181
Li
Analysis of the biosynthesis ...
Nocardiopsis alba, Nocardiopsis alba ATCC BAA-2165
Arch. Microbiol.
196
765-774
2014
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722021
Lautru
The albonoursin gene cluster o ...
Streptomyces noursei
Chem. Biol.
9
1355-1364
2002
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722172
Gondry
Cyclic dipeptide oxidase from ...
Streptomyces noursei, Streptomyces noursei ATCC 11455
Eur. J. Biochem.
268
1712-1721
2001
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