BRENDA - Enzyme Database
show all sequences of 1.3.3.15

Purification of and kinetic studies on a cloned protoporphyrinogen oxidase from the aerobic bacterium Bacillus subtilis

Corrigall, A.V.; Siziba, K.B.; Maneli, M.H.; Shephard, E.G.; Ziman, M.; Dailey, T.A.; Dailey, H.A.; Kirsch, R.E.; Meissner, P.N.; Arch. Biochem. Biophys. 358, 251-256 (1998)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
bilirubin
competitive inhibitor
Bacillus subtilis
Biliverdin
competitive inhibitor
Bacillus subtilis
hemin
competitive inhibitor
Bacillus subtilis
methylacifluorfen
weajk inhibition at 0.1 mM
Bacillus subtilis
additional information
the enzyme is not inhibited by the diphenyl ether herbicide acifluorfen at 0.1 mM
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
protoporphyrinogen IX
at pH 8.7 and 37°C
Bacillus subtilis
0.00429
-
mesoporphyrinogen IX
at pH 8.7 and 37°C
Bacillus subtilis
0.00529
-
Coproporphyrinogen III
at pH 8.7 and 37°C
Bacillus subtilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
x * 56000, SDS-PAGE
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
coproporphyrinogen III + O2
Bacillus subtilis
-
coproporphyrin III + H2O
-
-
?
mesoporphyrinogen IX + O2
Bacillus subtilis
-
mesoporphyrin IX + H2O
-
-
?
protoporphyrinogen IX + O2
Bacillus subtilis
-
protoporphyrin IX + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Bacillus subtilis
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni2+ affinity column chromatography
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
coproporphyrinogen III + O2
-
391011
Bacillus subtilis
coproporphyrin III + H2O
-
-
-
?
mesoporphyrinogen IX + O2
-
391011
Bacillus subtilis
mesoporphyrin IX + H2O
-
-
-
?
protoporphyrinogen IX + O2
-
391011
Bacillus subtilis
protoporphyrin IX + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 56000, SDS-PAGE
Bacillus subtilis
Synonyms
Synonyms
Commentary
Organism
hemY
-
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Bacillus subtilis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Bacillus subtilis
isoelectric focusing
-
7.5
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
bilirubin
competitive inhibitor
Bacillus subtilis
Biliverdin
competitive inhibitor
Bacillus subtilis
hemin
competitive inhibitor
Bacillus subtilis
methylacifluorfen
weajk inhibition at 0.1 mM
Bacillus subtilis
additional information
the enzyme is not inhibited by the diphenyl ether herbicide acifluorfen at 0.1 mM
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
protoporphyrinogen IX
at pH 8.7 and 37°C
Bacillus subtilis
0.00429
-
mesoporphyrinogen IX
at pH 8.7 and 37°C
Bacillus subtilis
0.00529
-
Coproporphyrinogen III
at pH 8.7 and 37°C
Bacillus subtilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
x * 56000, SDS-PAGE
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
coproporphyrinogen III + O2
Bacillus subtilis
-
coproporphyrin III + H2O
-
-
?
mesoporphyrinogen IX + O2
Bacillus subtilis
-
mesoporphyrin IX + H2O
-
-
?
protoporphyrinogen IX + O2
Bacillus subtilis
-
protoporphyrin IX + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+ affinity column chromatography
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
coproporphyrinogen III + O2
-
391011
Bacillus subtilis
coproporphyrin III + H2O
-
-
-
?
mesoporphyrinogen IX + O2
-
391011
Bacillus subtilis
mesoporphyrin IX + H2O
-
-
-
?
protoporphyrinogen IX + O2
-
391011
Bacillus subtilis
protoporphyrin IX + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 56000, SDS-PAGE
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Bacillus subtilis
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Bacillus subtilis
isoelectric focusing
-
7.5
Other publictions for EC 1.3.3.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737655
Hobbs
The HemQ coprohaem decarboxyla ...
Staphylococcus aureus
Biochem. J.
473
3997-4009
2016
-
-
1
-
-
-
-
1
-
-
-
2
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
739107
Lobo
Staphylococcus aureus haem bio ...
Staphylococcus aureus
Mol. Microbiol.
97
472-487
2015
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739547
Dailey
Noncanonical coproporphyrin-de ...
Bacillus subtilis
Proc. Natl. Acad. Sci. USA
112
2210-2215
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
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-
-
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2
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2
-
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-
-
-
-
-
-
-
-
712895
Qin
Structural insight into unique ...
Bacillus subtilis, Bacillus subtilis 168
J. Struct. Biol.
170
76-82
2010
-
-
1
1
5
-
1
-
1
-
-
4
-
3
-
-
1
-
-
-
-
-
4
1
2
-
-
-
-
-
-
-
1
1
-
-
-
-
1
1
1
5
-
-
1
1
-
1
-
-
4
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391011
Corrigall
Purification of and kinetic st ...
Bacillus subtilis
Arch. Biochem. Biophys.
358
251-256
1998
-
-
-
-
-
-
5
3
-
-
1
3
-
1
-
-
1
-
-
-
-
-
3
1
1
-
-
-
-
1
-
-
1
-
1
-
-
-
-
1
-
-
-
-
5
-
3
-
-
1
3
-
-
-
1
-
-
-
-
3
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
737734
Hansson
Isolated Bacillus subtilis Hem ...
Bacillus subtilis, Bacillus subtilis 168
Biochim. Biophys. Acta
1340
97-104
1997
-
-
1
-
-
-
-
-
-
-
1
4
-
3
-
-
1
-
-
-
-
-
4
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
4
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738501
Hansson
Bacillus subtilis HemY is a pe ...
Bacillus subtilis, Bacillus subtilis 3G18
J. Bacteriol.
176
5962-5970
1994
-
-
1
-
-
-
1
-
1
-
1
4
-
4
-
-
-
-
-
-
-
-
6
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
1
4
-
-
-
-
-
-
-
-
6
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738499
Hansson
Cloning and characterization o ...
Bacillus subtilis, Bacillus subtilis 3G18
J. Bacteriol.
174
8081-8093
1992
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-