Cloned (Comment) | Organism |
---|---|
gene AtCPO-1, chromosome I, DNA and amino acid sequence determination and analysis, complementation of an enzyme-deletion Saccharomyces cerevisiae hem13 mutant, expression as GFP-fusion protein in transgenic Arabidopsis thaliana plants via Agrobacterium tumefaciens tranbsformation | Arabidopsis thaliana |
gene AtCPO-2, DNA sequence determination and analysis, no complementation of an enzyme-deletion Saccharomyces cerevisiae hem13 mutant, possibly a pseudogene | Arabidopsis thaliana |
over 200fold overexpression in Escherichia coli strain BL21(DE3), reduction of the toxicity of product protoporphyrin-IX for the bacterial cells by decrease of growth temperature to 20°C | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
Coproporphyrinogen-III | recombinant enzyme | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
etioplast | - |
Glycine max | 9513 | - |
additional information | no activity in mitochondria | Glycine max | - |
- |
additional information | no activity in mitochondrion and cytosol | Arabidopsis thaliana | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Vigna radiata var. radiata |
37000 | - |
x * 37000, SDS-PAGE | Lotus japonicus |
37000 | - |
2 * 37000, recombinant and native wild-type enzyme, SDS-PAGE | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen-III + O2 | Glycine max | enzyme is involved in heme and chlorophyll biosynthesis | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
coproporphyrinogen-III + O2 | Arabidopsis thaliana | enzyme is involved in heme and chlorophyll biosynthesis | protoporphyrinogen-IX + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q93Z96 | 2 genesAtCPO-1 and AtCPO-2, the latter being a pseudogene | - |
Arabidopsis thaliana | Q9LR75 | 2 genesAtCPO-1 and AtCPO-2, the latter being a pseudogene | - |
Glycine max | - |
- |
- |
Lotus japonicus | - |
- |
- |
Vigna radiata var. radiata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli, 3-step procedure, 119fold to homogeneity | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Vigna radiata var. radiata | - |
leaf | - |
Lotus japonicus | - |
leaf | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
117.5 | - |
purified recombinant enzyme | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen-III + O2 | - |
Glycine max | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
coproporphyrinogen-III + O2 | - |
Arabidopsis thaliana | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
coproporphyrinogen-III + O2 | enzyme is involved in heme and chlorophyll biosynthesis | Glycine max | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
coproporphyrinogen-III + O2 | enzyme is involved in heme and chlorophyll biosynthesis | Arabidopsis thaliana | protoporphyrinogen-IX + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Vigna radiata var. radiata |
? | x * 37000, SDS-PAGE | Lotus japonicus |
dimer | 2 * 37000, recombinant and native wild-type enzyme, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
coproporphyrinogen III oxidase | - |
Vigna radiata var. radiata |
coproporphyrinogen III oxidase | - |
Glycine max |
coproporphyrinogen III oxidase | - |
Lotus japonicus |
coproporphyrinogen III oxidase | - |
Arabidopsis thaliana |
CPO | - |
Vigna radiata var. radiata |
CPO | - |
Glycine max |
CPO | - |
Lotus japonicus |
CPO | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |