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Literature summary for 1.3.3.4 extracted from

  • Camadro, J.M.; Thome, F.; Brouillet, N.; Labbe, P.
    Purification and properties of protoporphyrinogen oxidase from the yeast Saccharomyces cerevisiae: mitochondrial location and evidence for a precursor form of the protein (1994), J. Biol. Chem., 269, 32085-32091.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
fatty acid required for full activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
acifluorfen-methyl competitive Saccharomyces cerevisiae
additional information inhibited by diphenylether herbicides Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
protoporphyrinogen-IX
-
Saccharomyces cerevisiae
0.0005 0.0015 O2
-
Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion lipid bilayer of inner membrane Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrinogen-IX + 3 O2 Saccharomyces cerevisiae penultimate step of heme and chlorophyll biosynthesis protoporphyrin-IX + 3 H2O2
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.67
-
purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrinogen-IX + 3 O2
-
Saccharomyces cerevisiae protoporphyrin-IX + 3 H2O2
-
?
protoporphyrinogen-IX + 3 O2 penultimate step of heme and chlorophyll biosynthesis Saccharomyces cerevisiae protoporphyrin-IX + 3 H2O2
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
flavin
-
Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00001
-
acifluorfen-methyl
-
Saccharomyces cerevisiae