Literature summary for 1.3.3.5 extracted from

Xu, F.; Shin, W.; Brown, S.H.; Wahleithner, J.A.; Sundaram, U.M.; Solomon, E.I.
A study of a series of recombinant fungal laccases and bilirubin oxidase that exhibit significant differences in redox potential, substrate specificity, and stability (1996), Biochim. Biophys. Acta, 1292, 303-311.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Aspergillus oryzae	Albifimbria verrucaria

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.047	-	syringaldazine	at pH 8.2, lower Km at pH 7.0 and pH 9.0	Albifimbria verrucaria
0.076	-	2,2'-azino-bis(3-ethylbenzthioline-6-sulfonic acid)	at pH 2.7, Km increases with pH	Albifimbria verrucaria

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	3.7 copper atoms per enzyme molecule, recombinant enzyme	Albifimbria verrucaria

Organism

Organism	UniProt	Comment	Textmining
Albifimbria verrucaria	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Albifimbria verrucaria

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2	-	Albifimbria verrucaria	?	-	?
syringaldazine + O2	-	Albifimbria verrucaria	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 45000-50000, smear, SDS-PAGE	Albifimbria verrucaria

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
20	60	at pH 6.1	Albifimbria verrucaria
20	70	at pH 9.0	Albifimbria verrucaria
20	30	at pH 2.7	Albifimbria verrucaria

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0133	-	syringaldazine	at pH 5.3	Albifimbria verrucaria
2.08	-	syringaldazine	at about pH 8.0	Albifimbria verrucaria
7.17	-	2,2'-azino-bis(3-ethylbenzthioline-6-sulfonic acid)	at pH 5.3, about 3fold higher value at pH 2.5	Albifimbria verrucaria

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.7	-	oxidation of 2,2'-azino-bis(3-ethylbenzthioline-6-sulfonic acid)	Albifimbria verrucaria
8.2	-	oxidation of syringaldazine	Albifimbria verrucaria

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
2.7	9	-	Albifimbria verrucaria

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Release 2023.1 (January 2023)